Flavonoids are a large class of naturally occurring aromatic secondary plant metabolites.They constitute one of the most characteristic classes found in nature and morethan 4000 flavonoids have been identified and divided into several subclasses. Flavonoidshave several effects on human health, mainly related to their antioxidant activity.As a result of the increased interest in the biologically active compounds in food,many research studies have investigated the detection and quantification of antioxidants.Citrus plants are of great interest since they accumulate large amounts of flavonoidsand fruit and juices of these species are consumed in large quantities.Despite the extensive research performed on Citrus flavonoids, many compounds stillremain unidentified. In this work, flavonoids of five citrus juices: lemon, grapefruit,bergamot, orange, and mandarin were analyzed by microHPLC coupled on-line withan MS detector equipped with an ESI source operating in negative mode. The use ofmicrocolumn HPLC greatly enhanced detection performance. In total, 20 flavonoidsof two different subclasses (flavanone and flavone glycosides) were identified withthe help of retention data and MS spectral information.
Determination of flavonoids in citrus juices by micro-HPLC-ESI/MS
FAZIO, Alessia;
2005-01-01
Abstract
Flavonoids are a large class of naturally occurring aromatic secondary plant metabolites.They constitute one of the most characteristic classes found in nature and morethan 4000 flavonoids have been identified and divided into several subclasses. Flavonoidshave several effects on human health, mainly related to their antioxidant activity.As a result of the increased interest in the biologically active compounds in food,many research studies have investigated the detection and quantification of antioxidants.Citrus plants are of great interest since they accumulate large amounts of flavonoidsand fruit and juices of these species are consumed in large quantities.Despite the extensive research performed on Citrus flavonoids, many compounds stillremain unidentified. In this work, flavonoids of five citrus juices: lemon, grapefruit,bergamot, orange, and mandarin were analyzed by microHPLC coupled on-line withan MS detector equipped with an ESI source operating in negative mode. The use ofmicrocolumn HPLC greatly enhanced detection performance. In total, 20 flavonoidsof two different subclasses (flavanone and flavone glycosides) were identified withthe help of retention data and MS spectral information.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
