In our continuous search of biological properties of Senecio species (Compositae), we investigated S. ambiguus subsp. ambiguus (Biv.) DC, S. gibbosus subsp. gibbosus DC, S. leucanthemifolius Poiret, S. inaequidens DC, and S. vulgaris L. for their angiotensin converting enzyme (ACE) inhibitory activity through an in vitro bioassay based on the enzymatic cleavage of the chromophore-fluorophore labelled substrate dansyltriglycine into dansylglycine, which is quantitatively measured by HPLC. Among analyzed extracts, ethyl acetate demonstrated the highest activity with IC50 values of 192.1 and 219.1 μg/mL for S. ambiguus subsp. ambiguus and S. inaequidens, respectively. Flavonoids were detected in these extracts on TLC sprayed with Natural Products reagent - polyethylene glycal reagent (NP/PEG).
Inhibition of Angiotensin Converting Enzyme (ACE) Activity by Five Senecio Species (Compositae)
LOIZZO, Monica Rosa;TUNDIS, ROSA;CONFORTI, FILOMENA;
2009-01-01
Abstract
In our continuous search of biological properties of Senecio species (Compositae), we investigated S. ambiguus subsp. ambiguus (Biv.) DC, S. gibbosus subsp. gibbosus DC, S. leucanthemifolius Poiret, S. inaequidens DC, and S. vulgaris L. for their angiotensin converting enzyme (ACE) inhibitory activity through an in vitro bioassay based on the enzymatic cleavage of the chromophore-fluorophore labelled substrate dansyltriglycine into dansylglycine, which is quantitatively measured by HPLC. Among analyzed extracts, ethyl acetate demonstrated the highest activity with IC50 values of 192.1 and 219.1 μg/mL for S. ambiguus subsp. ambiguus and S. inaequidens, respectively. Flavonoids were detected in these extracts on TLC sprayed with Natural Products reagent - polyethylene glycal reagent (NP/PEG).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.