In a single-step free radical reaction, multicatalytic hydrogels were synthesized by covalent immobilization of Pancreatin onto a film composed of Acrylamide and Polyethylene glycol dimethacrylate750. Hydrogels were characterized by determination of their dynamic swelling ratios and each catalytic activity was extensively investigated by determination of kinetic parameters KM and Vmax. The immobilization process was found to preserve the hydrolytic properties of Pancreatin (Protease, Lipase, and Amylase catalytic activities). Catalytic efficiencies were the highest with Protease and the lowest with Amylase. Reusability values higher than 60% after 10 repeated cycles proved the applicability of the proposed material in industrial practice.
Functional hydrogels with a multicatalytic activity for bioremediation: Single-step preparation and characterization
Spizzirri, UG;Curcio, M;Cirillo, G;Picci, N;Nicoletta, FP;Iemma, F
2016-01-01
Abstract
In a single-step free radical reaction, multicatalytic hydrogels were synthesized by covalent immobilization of Pancreatin onto a film composed of Acrylamide and Polyethylene glycol dimethacrylate750. Hydrogels were characterized by determination of their dynamic swelling ratios and each catalytic activity was extensively investigated by determination of kinetic parameters KM and Vmax. The immobilization process was found to preserve the hydrolytic properties of Pancreatin (Protease, Lipase, and Amylase catalytic activities). Catalytic efficiencies were the highest with Protease and the lowest with Amylase. Reusability values higher than 60% after 10 repeated cycles proved the applicability of the proposed material in industrial practice.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
