The sequence of isomeric dinucleotides, bearing 5'or 3'-terminal phosphate groups, which is not correlated to the proton affinity of the nucleobases, is discussed. The structural determination of dinucleotides by mass spectrometry (MS) follows a simple rule that involves the fragmentation of the parent ion [M-H] - in a MS/MS experiment. The [M-H] - parent ion possess a zwitterionic structure in which the negative charge is placed on a phosphate group. The low energy collisions on the [M-H] - precursor ions indicate that internucleotidic bond dissociation is directed by the position of the terminal group.
The sequence of isomeric dinucleotides, bearing 5′ or 3′-terminal phosphate groups, is not correlated to the proton affinity of the nucleobases
DI DONNA, Leonardo;NAPOLI, Anna Maria Carmela Natale V;
2002-01-01
Abstract
The sequence of isomeric dinucleotides, bearing 5'or 3'-terminal phosphate groups, which is not correlated to the proton affinity of the nucleobases, is discussed. The structural determination of dinucleotides by mass spectrometry (MS) follows a simple rule that involves the fragmentation of the parent ion [M-H] - in a MS/MS experiment. The [M-H] - parent ion possess a zwitterionic structure in which the negative charge is placed on a phosphate group. The low energy collisions on the [M-H] - precursor ions indicate that internucleotidic bond dissociation is directed by the position of the terminal group.File in questo prodotto:
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