The search of natural products for cancer therapy represents an area of great interest in which plants have been the most important source. In the continuing search for cytotoxic compounds from plants in the present investigation we reported the cytotoxic activity of extracts from S. oppositifolia against CORL-23 and C-32 tumor cell lines. The Salsola genus (Amaranthaceae) comprises about 120 species; they are very diffused annual herbaceous plants, especially in the brackish grounds of the moderate and subtropical regions of Europe, Asia, Africa and north America. Salsola species are well known in traditional medicine and nowadays have been widely used with a large variety of beneficial effects, such as anticancer, diuretic, anti-hypertensive, and anti-inflammatory [1-4]. Dried aerial parts were extracted with MeOH at room temperature. The extract was dissolved in a MeOH/H2O (9:1) mixture and partitioned with n-hexane, dichloromethane, and ethyl acetate. The cytotoxicity was evaluated using the sulforodamine B (SRB) assay [5]. The test is based on the estimation of cell number indirectly by providing a sensitive index of total cellular protein content which is linear to cell density. The most active extracts to inhibit proliferation of non-small lung carcinoma cell line were the dichloromethane and n-hexane extracts with IC50 values of 31.90 μg/mL and 19.09 μg/mL, respectively. The dichloromethane extract was able also to inhibit proliferation of melanoma cell line with IC50 value of 36.29 μg/mL. The chemical composition of the active extracts was reported. References: 1.Al-Saleh, F.S., et al. (1993) Fitoterapia LXIV 3: 251-256. 2.Nikiforov S.B., et al. (2002) Pharm. Chem. J. 36:544-554. 3.Beloborodova E.I., et al. (2000) Klin. Med. 78:56-9. 4.Fu S. (1959) Zhonghua Nei Ke Za Zhi, 7:977-981. 5. Monks, A. et al. (1991) J. Nat. Cancer Institute, 83:757-66.
Cytotoxic extracts of Salsola oppositifolia Desf. (Amaranthaceae) against non-small lung carcinoma (CORL-23) and melanoma (C32) cells
TUNDIS, ROSA;LOIZZO, Monica Rosa;BONESI M;CONFORTI, FILOMENA;
2007-01-01
Abstract
The search of natural products for cancer therapy represents an area of great interest in which plants have been the most important source. In the continuing search for cytotoxic compounds from plants in the present investigation we reported the cytotoxic activity of extracts from S. oppositifolia against CORL-23 and C-32 tumor cell lines. The Salsola genus (Amaranthaceae) comprises about 120 species; they are very diffused annual herbaceous plants, especially in the brackish grounds of the moderate and subtropical regions of Europe, Asia, Africa and north America. Salsola species are well known in traditional medicine and nowadays have been widely used with a large variety of beneficial effects, such as anticancer, diuretic, anti-hypertensive, and anti-inflammatory [1-4]. Dried aerial parts were extracted with MeOH at room temperature. The extract was dissolved in a MeOH/H2O (9:1) mixture and partitioned with n-hexane, dichloromethane, and ethyl acetate. The cytotoxicity was evaluated using the sulforodamine B (SRB) assay [5]. The test is based on the estimation of cell number indirectly by providing a sensitive index of total cellular protein content which is linear to cell density. The most active extracts to inhibit proliferation of non-small lung carcinoma cell line were the dichloromethane and n-hexane extracts with IC50 values of 31.90 μg/mL and 19.09 μg/mL, respectively. The dichloromethane extract was able also to inhibit proliferation of melanoma cell line with IC50 value of 36.29 μg/mL. The chemical composition of the active extracts was reported. References: 1.Al-Saleh, F.S., et al. (1993) Fitoterapia LXIV 3: 251-256. 2.Nikiforov S.B., et al. (2002) Pharm. Chem. J. 36:544-554. 3.Beloborodova E.I., et al. (2000) Klin. Med. 78:56-9. 4.Fu S. (1959) Zhonghua Nei Ke Za Zhi, 7:977-981. 5. Monks, A. et al. (1991) J. Nat. Cancer Institute, 83:757-66.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
