Diabetes is defined as a state in which homeostasis of carbohydrate and lipid metabolism is improperly regulated by the pancreatic hormone, insulin; resulting in an increased blood glucose level. World Health Organization (WHO) suggests that the number of cases for diabetes that is currently at 171 million is predicted to reach 366 million by the year 2030 (1). Citrus medica L. cv. Diamante is the cultivar more diffused in Italy and more sought by industry. The peel of Citrus fruits has been used in traditional Asian medicine for centuries (2,3). C. medica var. Diamante peel 70% hydroalcoholic extract analyzed by HPLC revealed the presence of apigenin, hesperitin, naringin quercetin and rutin as main constituent. The alpha-amylase activity measured by in vitro assay (4) showed an IC50 value of 1.72 mg/mL. Moreover, peel extract caused a significant increase in insulin secretion from MIN6 cells grown as monolayers and as pseudoislets (5). C. medica initiated a concentration-dependent stimulation of insulin secretion at 2 mM glucose. Exposure of pseudoislet to 1 mg/mL of C. medica var. Diamante extract resulted in a rapid enhancement of the secretory responses to glucose. These results indicate that the antidiabetic activity may be as a result of increased insulin secretion by pancreatic islet cells. This works suggest the potential use of C. medica var. Diamante peel extract as food or nutriceutical products with particular relevance to supplements for diabetic patients.
In vitro effect of Citrus medica var. Diamante peel extract on enzyme involved in diabetes and on insulin secretion
LOIZZO, Monica Rosa;TUNDIS, ROSA;
2009-01-01
Abstract
Diabetes is defined as a state in which homeostasis of carbohydrate and lipid metabolism is improperly regulated by the pancreatic hormone, insulin; resulting in an increased blood glucose level. World Health Organization (WHO) suggests that the number of cases for diabetes that is currently at 171 million is predicted to reach 366 million by the year 2030 (1). Citrus medica L. cv. Diamante is the cultivar more diffused in Italy and more sought by industry. The peel of Citrus fruits has been used in traditional Asian medicine for centuries (2,3). C. medica var. Diamante peel 70% hydroalcoholic extract analyzed by HPLC revealed the presence of apigenin, hesperitin, naringin quercetin and rutin as main constituent. The alpha-amylase activity measured by in vitro assay (4) showed an IC50 value of 1.72 mg/mL. Moreover, peel extract caused a significant increase in insulin secretion from MIN6 cells grown as monolayers and as pseudoislets (5). C. medica initiated a concentration-dependent stimulation of insulin secretion at 2 mM glucose. Exposure of pseudoislet to 1 mg/mL of C. medica var. Diamante extract resulted in a rapid enhancement of the secretory responses to glucose. These results indicate that the antidiabetic activity may be as a result of increased insulin secretion by pancreatic islet cells. This works suggest the potential use of C. medica var. Diamante peel extract as food or nutriceutical products with particular relevance to supplements for diabetic patients.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.