Purpose: To present an optical characterization method for measuring time-dependent corneo-sclera shell biomechanics, through an integration of Electronic Laser Speckle Interferometry (ESPI) and 3D geometry reconstruction. Methods: The posterior pole of a human donor eye has been inflation tested to record the time-varying displacement and strain fields in the peripapillary and mid-peripheral sclera. The pressure inside the eye was abruptly elevated by manually switching pressure between two PBS reservoirs set at 5 and 30 mmHg (IOP-transient test). The anterior pole from the same donor eye was later inflation tested by steadily increasing the IOP for the same pressure range. A custom-built ESPI composed of a laser and four synchronized cameras simultaneously recorded the time-varying deformations with nanometric accuracy (<30 nm) and an imaging rate of 180Hz. The multi-camera optical set-up allowed for a 3D stereo geometry reconstruction of the specimen shapes with an accuracy lower than 25 μm. Results: The IOP elevation in the cornea generated high values of strain in the limbus region, close to the apex, and around muscle insertions (Fig. 1, right). In the sclera, high gradients of strain were observed around the optic nerve but not in the mid-peripheral region (Fig.1, left). In the IOP-transient test the peripapillary and mid-peripheral sclera showed a similar overall average deformation rate during the IOP rapid increase (1000<time<2500 µs), while once relaxation was reached (time>3000 µs) the peripapillary sclera showed higher displacements than the mid-peripheral. Conclusions: Thanks to the high rate interferometric imaging, the dynamic response of the corneo-sclera shell can be finely resolved in both the spatial and temporal domain. This will allow for the investigation of the corneo-sclera mechanical response to time-varying IOP, and how its biomechanics is altered in ocular diseases like glaucoma, myopia, and keratoconus.

Characterization method for the IOP-time-dependent cornea and sclera biomechanics

Bianco G
;
BRUNO, LUIGI;Fazio MA
2017

Abstract

Purpose: To present an optical characterization method for measuring time-dependent corneo-sclera shell biomechanics, through an integration of Electronic Laser Speckle Interferometry (ESPI) and 3D geometry reconstruction. Methods: The posterior pole of a human donor eye has been inflation tested to record the time-varying displacement and strain fields in the peripapillary and mid-peripheral sclera. The pressure inside the eye was abruptly elevated by manually switching pressure between two PBS reservoirs set at 5 and 30 mmHg (IOP-transient test). The anterior pole from the same donor eye was later inflation tested by steadily increasing the IOP for the same pressure range. A custom-built ESPI composed of a laser and four synchronized cameras simultaneously recorded the time-varying deformations with nanometric accuracy (<30 nm) and an imaging rate of 180Hz. The multi-camera optical set-up allowed for a 3D stereo geometry reconstruction of the specimen shapes with an accuracy lower than 25 μm. Results: The IOP elevation in the cornea generated high values of strain in the limbus region, close to the apex, and around muscle insertions (Fig. 1, right). In the sclera, high gradients of strain were observed around the optic nerve but not in the mid-peripheral region (Fig.1, left). In the IOP-transient test the peripapillary and mid-peripheral sclera showed a similar overall average deformation rate during the IOP rapid increase (10003000 µs) the peripapillary sclera showed higher displacements than the mid-peripheral. Conclusions: Thanks to the high rate interferometric imaging, the dynamic response of the corneo-sclera shell can be finely resolved in both the spatial and temporal domain. This will allow for the investigation of the corneo-sclera mechanical response to time-varying IOP, and how its biomechanics is altered in ocular diseases like glaucoma, myopia, and keratoconus.
Dynamic deformations
Cornea biomechanics
Sclera biomechanics
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11770/175163
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