Cultivation of Citrus medica L. cv. Diamante and Citrus bergamia Risso species are limited to Calabria, Southern Italy. The n-hexane peel extracts of both species were analysed by GC-MS. The Diamante citron peel extract is characterized by the presence of four monoterpenes as main components: limonene, γ-terpinene, nerol and geraniol. The sesquiterpene fraction was less represented; the major constituents were -bisabolene and -bergamotene. The coumarin citropten was also found. C. bergamia peel was characterized by the presence of monoterpenes linalool, linalyl acetate, limonene, γ-terpinene, -pinene and -pinene and the coumarin bergaptene. Antioxidant activity was measured trough the DPPH assay [2]. C. medica peel extract showed an IC50 of 147 μg/mL while low activity was found when C. bergamia peel extract was used. Inhibition of AchE was assessed by a modified colorimetric method of Ellman [4]. C. medica L. cv. Diamante peel extract exerted an interesting activity against AchE with an IC50 of 621 μg/mL. On the contrary the same extract obtained from C. bergamia showed less activity with IC50 of 5.02 mg/mL. Limonene, the most abundant compound of C. medica L. cv. Diamante peel extract, showed an inhibition of 22.6% at 1.2 mM. References: 1.Conforti, F. et al. (2005) Biol. Pharm. Bull. 28:1791. 2.Perry, N.S. et al. (1992) J. Pharm. Pharmacol. 52:895.
Comparative chemical composition, antioxidant activity and acetylcholinesterase inhibition of Citrus medica L. cv. Diamante and Citrus bergamia Risso
LOIZZO, Monica Rosa;TUNDIS, ROSA;BONESI M;CONFORTI, FILOMENA;
2007-01-01
Abstract
Cultivation of Citrus medica L. cv. Diamante and Citrus bergamia Risso species are limited to Calabria, Southern Italy. The n-hexane peel extracts of both species were analysed by GC-MS. The Diamante citron peel extract is characterized by the presence of four monoterpenes as main components: limonene, γ-terpinene, nerol and geraniol. The sesquiterpene fraction was less represented; the major constituents were -bisabolene and -bergamotene. The coumarin citropten was also found. C. bergamia peel was characterized by the presence of monoterpenes linalool, linalyl acetate, limonene, γ-terpinene, -pinene and -pinene and the coumarin bergaptene. Antioxidant activity was measured trough the DPPH assay [2]. C. medica peel extract showed an IC50 of 147 μg/mL while low activity was found when C. bergamia peel extract was used. Inhibition of AchE was assessed by a modified colorimetric method of Ellman [4]. C. medica L. cv. Diamante peel extract exerted an interesting activity against AchE with an IC50 of 621 μg/mL. On the contrary the same extract obtained from C. bergamia showed less activity with IC50 of 5.02 mg/mL. Limonene, the most abundant compound of C. medica L. cv. Diamante peel extract, showed an inhibition of 22.6% at 1.2 mM. References: 1.Conforti, F. et al. (2005) Biol. Pharm. Bull. 28:1791. 2.Perry, N.S. et al. (1992) J. Pharm. Pharmacol. 52:895.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
