Chemical engineers contribute to society through the useful application of knowledge and understanding of chemistry, physics, biology, and mathematics. Emerging new areas such as biotechnology, nanofabrication technology, semiconductor devices and modern construction materials also utilize the unique capabilities of the chemical engineer. On the global scale, liver diseases are severe public health problems, with the incidences of end-stage liver disease (ESLD) rising annually. The impairment of liver functions has also serious implications and it is responsible for high rates of patient morbidity and mortality. An important challenge in engineering devices for culturing hepatocytes is the development of bioartificial systems that are able to favour the liver reconstruction and to modulate liver cell behaviour. Bioreactors allow the culture of cells under tissue specific mechanical forces (e.g. pressure, shear stress and interstitial flow), augmenting the gas and nutrient exchange under fluid dynamics control that ensure the long-term maintenance of cell viability and functions. However, the increasing complexity of fluid dynamics and transport phenomena in present and future bioreactors requires advanced steady-state models and control strategies for the transient operations, since a well-controlled environment with respect to transfer processes and metabolic kinetics is necessary for activation of specific cellular response and long-time viability. In this work, Stable and durable operation of bioartificial reactors for cell cultures is investigated. More specifically, tight control of the culturing environment and strategies for dealing with some inherently unsteady changes of conditions (e.g. fouling) in a membrane bioreactor is investigated. In this poster, Preliminary results of this work will be described later. Then, efforts will be devoted to set-up a more accurate dynamic model of the process able to contemplate all the key aspects determining a successful cell culturing process (transport of nutrients, catabolites, control of oxygen flow, liquid level, temperature and pH levels etc.). Such a model will constitute the basis for the development of a full, multi-variable control loop. The second part of the work involves selecting appropriate sensors for on-line monitoring of relevant properties as well as the manipulated variables for control. Different control schemes will be tested by making use of specific control system software packages. Ultimately, the present work will allow improving the understanding of the behaviour of bioreactor for biomedical application under transient conditions, as well as gaining the ability proactively increase the performances ( both during operations and in terms of cell culture durability) by means of specific automation and control techniques.
Development of process control strategies for cell culture in membrane bioreactor
DI RENZO A;CURCIO, EFREM
2014-01-01
Abstract
Chemical engineers contribute to society through the useful application of knowledge and understanding of chemistry, physics, biology, and mathematics. Emerging new areas such as biotechnology, nanofabrication technology, semiconductor devices and modern construction materials also utilize the unique capabilities of the chemical engineer. On the global scale, liver diseases are severe public health problems, with the incidences of end-stage liver disease (ESLD) rising annually. The impairment of liver functions has also serious implications and it is responsible for high rates of patient morbidity and mortality. An important challenge in engineering devices for culturing hepatocytes is the development of bioartificial systems that are able to favour the liver reconstruction and to modulate liver cell behaviour. Bioreactors allow the culture of cells under tissue specific mechanical forces (e.g. pressure, shear stress and interstitial flow), augmenting the gas and nutrient exchange under fluid dynamics control that ensure the long-term maintenance of cell viability and functions. However, the increasing complexity of fluid dynamics and transport phenomena in present and future bioreactors requires advanced steady-state models and control strategies for the transient operations, since a well-controlled environment with respect to transfer processes and metabolic kinetics is necessary for activation of specific cellular response and long-time viability. In this work, Stable and durable operation of bioartificial reactors for cell cultures is investigated. More specifically, tight control of the culturing environment and strategies for dealing with some inherently unsteady changes of conditions (e.g. fouling) in a membrane bioreactor is investigated. In this poster, Preliminary results of this work will be described later. Then, efforts will be devoted to set-up a more accurate dynamic model of the process able to contemplate all the key aspects determining a successful cell culturing process (transport of nutrients, catabolites, control of oxygen flow, liquid level, temperature and pH levels etc.). Such a model will constitute the basis for the development of a full, multi-variable control loop. The second part of the work involves selecting appropriate sensors for on-line monitoring of relevant properties as well as the manipulated variables for control. Different control schemes will be tested by making use of specific control system software packages. Ultimately, the present work will allow improving the understanding of the behaviour of bioreactor for biomedical application under transient conditions, as well as gaining the ability proactively increase the performances ( both during operations and in terms of cell culture durability) by means of specific automation and control techniques.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
