The effect of hypothyroidism, induced by 6-n-propyl-2-thiouracil administration to rats, on the retinoylation reaction was investigated in rat-testes mitochondria. In hypothyroid mitochondria, compared to euthyroid controls, we found an increase of the all-trans-retinoic acid (RA) bound to mitochondrial proteins by an acylation process (34.2 pmoles RA/mg protein/360 min and 22.2 pmoles RA/mg protein/360 min). This increase, which was time- and temperature-dependent, was accompanied by a strong reduction in the cardiolipin (CL) amount in the mitochondrial membranes of hypothyroid (2.6%) as compared to euthyroid rats (4.5%). Conversely, a decreased retinoylation reaction was observed when CL liposomes were added to mitochondria or mitoplasts from both euthyroid and hypothyroid rats, confi rming a role of CL in the retinoylation process. The ATP level, which was reduced in hypothyroid mitochondria (27.3 pmoles ATP/mg protein versus 67.1 pmoles ATP/mg protein of euthyroid animals), was surprisingly increased in mitochondria by the retinoylation reaction in the presence of 100 nM RA (481.5 pmoles ATP/mg protein of hypothyroid rats versus 84.7 pmoles ATP/mg protein of euthyroid rats).

Effect of cardiolipin on retinoylation reaction in testes mitochondria from hypothyroid rats

LAURIA, GRAZIANTONIO;
2010-01-01

Abstract

The effect of hypothyroidism, induced by 6-n-propyl-2-thiouracil administration to rats, on the retinoylation reaction was investigated in rat-testes mitochondria. In hypothyroid mitochondria, compared to euthyroid controls, we found an increase of the all-trans-retinoic acid (RA) bound to mitochondrial proteins by an acylation process (34.2 pmoles RA/mg protein/360 min and 22.2 pmoles RA/mg protein/360 min). This increase, which was time- and temperature-dependent, was accompanied by a strong reduction in the cardiolipin (CL) amount in the mitochondrial membranes of hypothyroid (2.6%) as compared to euthyroid rats (4.5%). Conversely, a decreased retinoylation reaction was observed when CL liposomes were added to mitochondria or mitoplasts from both euthyroid and hypothyroid rats, confi rming a role of CL in the retinoylation process. The ATP level, which was reduced in hypothyroid mitochondria (27.3 pmoles ATP/mg protein versus 67.1 pmoles ATP/mg protein of euthyroid animals), was surprisingly increased in mitochondria by the retinoylation reaction in the presence of 100 nM RA (481.5 pmoles ATP/mg protein of hypothyroid rats versus 84.7 pmoles ATP/mg protein of euthyroid rats).
2010
Retinoylation reaction; Cardiolipin; Testes mitochondria; Hypothyroid rats
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11770/182850
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