Inhibition of Angiotensin-Converting Enzyme of Salsola kali L. and Salsola soda

Inhibition of Angiotensin Converting Enzyme (ACE) is currently considered to be a useful therapeutic approach in the treatment of high blood pressure (1). Inhibition of ACE will be an effective screening method in the serch for new antihypertensive agents (2). This study deals with the investigation of the ACE inhibitory properties. of extracts of S. kali L. and S. soda L. aerial parts. Dried aerial parts of Salsola kali L. (212.92 g) and Salsola soda L. (291.63 g) were extracted with MeOH at room temperature. The combined MeOH solution were concentrated under reduced pressure. A solid residue was precipitated and separated by filtration from S. kali extract upon cooling. Both extracts were dissolved in a MeOH/H2O (9:1) mixture and partitioned with n-hexane, dichloromethane, ethyl acetate and ethyl acetate after basification with NH4OH. The evaluation of activity was investigated by the method based on the ACE-catalyzed cleavage of the chromophore-fluorophore labelled substrate dansyltriglycine into dansylglycine, which is quantitatively measured by HPLC (3). Solutions of inhibitors were made by dissolving 1 mg of extract test in 1 ml HEPES assay buffer, to obtain the final concentration of 330 μg/ml. The most effective fraction of S. soda is the EtOAc extract, which gave 55.87 % inhibition at 330 μg/ml. Also the S. kali EtOAc extract exhibited ACE inhibition activity with a value of 36.21 % at the same concentration. References: 1. Lacaille-Dubois, M.A. et al. (2001) Phytomedicine 8: 47-52. 2. Wagner, H. (1993) J. Ethnopharmacol. 38: 105-112. 3. Elbl, G., Wagner, H. (1991) Planta Medica 57: 137-141.