The antiproliferative activity of several flavonoids isolated from Linaria reflexa Desf. (Scrophulariaceae) was evaluated in vitro by SRB assay1 against the large cell lung carcinoma cell line COR-L23, the hepatocellular carcinoma cell line HepG-2, the renal cell adenocarcinoma ACHN, the amelanotic melanoma cell line C32, and the normal human fetal lung MRC5. Chemical modification, that is acetylation, hydrolysis of rutinose unit, and hydrolysis of the terminal rhamnose unit, were performed on pectolinarin2-4. Pectolinarin exhibited strong cytotoxic activity on C32 and COR-L23 cell lines with an IC50 of 7.17 uM and 5.03 uM, respectively. Similar activities were recorded for the three natural monoacetyl pectolinarin derivatives linariin, isolinariin A, isolinariin B. In contrast, peracetylpectolinarin only displayed marginal activity. 1. Skehan P., Storeng R., Scudiero D., Monks A., McMahon I., Vistica D., Waren J.T., Bokesch H., Kenney S., Boyd M.R., J. Nat. Cancer Inst., 1990, 82, 1107-1111. 2. Hase T., Ohtani K., Kasai R., Yamasaki K., Picheansoonthon C., Phytochemistry, 1995, 40, 287-290. 3. Farag S.F., Backheet E.Y., El-Emary N.A., Niwa M., Phytochemistry, 1999, 50, 1407-1410. 4. Mahato S.B., Sahu N.P., Roy S.K., Sharma O.P., Tetrahedron, 1994, 50, 9439-9446.
Cytotoxic activity of flavones and their derivatives from Linaria reflexa Desf
TUNDIS, ROSA;LOIZZO, Monica Rosa;M. BONESI;
2005-01-01
Abstract
The antiproliferative activity of several flavonoids isolated from Linaria reflexa Desf. (Scrophulariaceae) was evaluated in vitro by SRB assay1 against the large cell lung carcinoma cell line COR-L23, the hepatocellular carcinoma cell line HepG-2, the renal cell adenocarcinoma ACHN, the amelanotic melanoma cell line C32, and the normal human fetal lung MRC5. Chemical modification, that is acetylation, hydrolysis of rutinose unit, and hydrolysis of the terminal rhamnose unit, were performed on pectolinarin2-4. Pectolinarin exhibited strong cytotoxic activity on C32 and COR-L23 cell lines with an IC50 of 7.17 uM and 5.03 uM, respectively. Similar activities were recorded for the three natural monoacetyl pectolinarin derivatives linariin, isolinariin A, isolinariin B. In contrast, peracetylpectolinarin only displayed marginal activity. 1. Skehan P., Storeng R., Scudiero D., Monks A., McMahon I., Vistica D., Waren J.T., Bokesch H., Kenney S., Boyd M.R., J. Nat. Cancer Inst., 1990, 82, 1107-1111. 2. Hase T., Ohtani K., Kasai R., Yamasaki K., Picheansoonthon C., Phytochemistry, 1995, 40, 287-290. 3. Farag S.F., Backheet E.Y., El-Emary N.A., Niwa M., Phytochemistry, 1999, 50, 1407-1410. 4. Mahato S.B., Sahu N.P., Roy S.K., Sharma O.P., Tetrahedron, 1994, 50, 9439-9446.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
