The sequence of isomeric dinucleotides, bearing 5'or 3'-terminal phosphate groups, which is not correlated to the proton affinity of the nucleobases, is discussed. The structural determination of dinucleotides by mass spectrometry (MS) follows a simple rule that involves the fragmentation of the parent ion [M-H]- in a MS/MS experiment. The [M-H]- parent ion possess a zwitterionic structure in which the negative charge is placed on a phosphate group. The low energy collisions on the [M-H]- precursor ions indicate that internucleotidic bond dissociation is directed by the position of the terminal group.

The sequence of isomeric dinucleotides, bearing 5′ or 3′-terminal phosphate groups, is not correlated to the proton affinity of the nucleobases

Di Donna, Leonardo;Napoli, Anna;Sindona, Giovanni;
2002

Abstract

The sequence of isomeric dinucleotides, bearing 5'or 3'-terminal phosphate groups, which is not correlated to the proton affinity of the nucleobases, is discussed. The structural determination of dinucleotides by mass spectrometry (MS) follows a simple rule that involves the fragmentation of the parent ion [M-H]- in a MS/MS experiment. The [M-H]- parent ion possess a zwitterionic structure in which the negative charge is placed on a phosphate group. The low energy collisions on the [M-H]- precursor ions indicate that internucleotidic bond dissociation is directed by the position of the terminal group.
Spectroscopy
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11770/275043
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