The thermal denaturation of plastocyanin in aqueous solution was investigated by means of DSC, ESR and absorbance techniques, with the aim of determining the thermodynamic stability of the protein and of characterizing the thermally induced conformational changes of its active site. The DSC and absorbance experiments indicated an irreversible and kinetically controlled denaturation path. The extrapolation of the heat capacity and optical data at infinite scan rate made it possible to calculate the kinetic and thermodynamic parameters associated with the denaturation steps. The denaturation pathway proposed, and the parameters found from the calorimetric data, were checked by computer simulation using an equation containing the information necessary to describe the denaturation process in detail. ESR and absorbance measurements have shown that structural changes of the copper environment occur during the protein denaturation. In particular, the geometry of the copper-ligand atoms changes from being tetrahedral to square planar and the disruption of the active site precedes the global protein denaturation. The thermodynamic enthalpic change, the half-width transition temperature, and the value of DCp, were used to calculate the thermodynamic stability, DG, of the reversible process over the entire temperature range of denaturation. The low thermal stability found for plastocyanin, is discussed in connection with structural factors stabilizing the native state of a protein.
Thermodynamics and kinetcs of the thermal unfolding of plastocyanin / Milardi, D.; LA ROSA, C.; Grasso, D.; Guzzi, Rita; Sportelli, L.; Fini, C.. - In: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. - ISSN 0175-7571. - 27(1998), pp. 273-282.
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|Titolo:||Thermodynamics and kinetcs of the thermal unfolding of plastocyanin|
|Data di pubblicazione:||1998|
|Citazione:||Thermodynamics and kinetcs of the thermal unfolding of plastocyanin / Milardi, D.; LA ROSA, C.; Grasso, D.; Guzzi, Rita; Sportelli, L.; Fini, C.. - In: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. - ISSN 0175-7571. - 27(1998), pp. 273-282.|
|Appare nelle tipologie:||1.1 Articolo in rivista|