Extracts of Senecio samnitum Huet and derived methylester of chlorogenic acid have been shown to inhibitangiotensin-converting enzyme (ACE) by using anin vitro bioassay based on the enzymatic cleavage ofthe chromophore-fluorophore labeled substrate dansyltriglycineinto dansylglycine, which is quantitativelymeasured by high-performance liquid chromatography(HPLC). GC-MS and NMR identified compoundspresent within the studied S. samnitum extracts. Themost effective fraction was obtained in ethyl acetate,which gave 52.56 0.23% (SD) inhibition at 300 ug/ml.The major constituent of this fraction, the methyl esterof chlorogenic acid, showed significant ACE inhibitionof 56.78 at a concentration of 82.5 ug/ml.
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|Titolo:||Chemical composition and inhibition of angiotensin-converting enzyme of Senecio samnitum Huet|
|Data di pubblicazione:||2005|
|Appare nelle tipologie:||1.1 Articolo in rivista|