The present study was conducted to assess the toxicity of Foroozan (Iranian crude oil) on the ornate wrasse Thalassomapavo (Labridae) using three sub-lethal crude oil concentrations. Gills, selected as target organ being the major route ofhydrocarbon uptake, were excised after 48, 96 and 192 hours and analyzed by light and both scanning and transmissionelectron microscopy. Cellular biomarkers of exposure and/or effect were measured in branchial tissues of T. pavo, andsevere lesions such as necrosis, aneurysms and disorganization of the lamellae proportional to the Foroozan exposure periodsand concentrations were observed. The main alteration was on the lamellar epithelium, which displayed an irregularprofile characterized by pavement cell cytoplasmic protrusion and an alteration of the oxygen chemosensing cells, resultingin impairment of various biological activities. Nevertheless, the ability of specimens to regulate calcium homeostasis andneurotransmission, as well as balance cell turnover, suggests that the species tested to not only survive but also recover insuch high crude oil dosage regimen. The identification in gills of histological and neurological changes associated with acutecrude oil exposure confirms the utility of the sub-lethal toxicity tests.
Toxicity of Foroozan crude oil to ornate wrasse (Thalassoma pavo, Osteichthyes, Labridae): ultrastructure and cellular biomarkers
Sperone E;Bernabo' I;TRIPEPI, Sandro;BRUNELLI, Elvira
2012-01-01
Abstract
The present study was conducted to assess the toxicity of Foroozan (Iranian crude oil) on the ornate wrasse Thalassomapavo (Labridae) using three sub-lethal crude oil concentrations. Gills, selected as target organ being the major route ofhydrocarbon uptake, were excised after 48, 96 and 192 hours and analyzed by light and both scanning and transmissionelectron microscopy. Cellular biomarkers of exposure and/or effect were measured in branchial tissues of T. pavo, andsevere lesions such as necrosis, aneurysms and disorganization of the lamellae proportional to the Foroozan exposure periodsand concentrations were observed. The main alteration was on the lamellar epithelium, which displayed an irregularprofile characterized by pavement cell cytoplasmic protrusion and an alteration of the oxygen chemosensing cells, resultingin impairment of various biological activities. Nevertheless, the ability of specimens to regulate calcium homeostasis andneurotransmission, as well as balance cell turnover, suggests that the species tested to not only survive but also recover insuch high crude oil dosage regimen. The identification in gills of histological and neurological changes associated with acutecrude oil exposure confirms the utility of the sub-lethal toxicity tests.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
