The object of the study was to determine thechemical composition of Diospyros lotus L. extract andtheir antioxidant and antiproliferative properties. Eight compounds were isolated from D. lotus and identified as gallic acid, methylgallate, ellagic acid, kaempferol, quercetin,myricetin, myricetin 3-O-β-glucuronide, and myricetin-3-O-α-rhamnoside. D. lotus extract tested in different invitro systems (DPPH, ABTS, FRAP, and Fe2+ chelatingactivity assay) showed significant antioxidant activity. Thepotential antiproliferative properties of D. lotus extract andisolated compounds against nine human cancer cell linessuch as COR-L23, CaCo-2, C32, ACHN, A375, A549,Huh-7D12, MCF-7, and LNCaP were investigated in vitroby SRB assay. D. lotus extract demonstrated the highestinhibitory activity against COR-L23 with an IC50 value of12.2 μg/ml. Among identified hydrolysable tannins, ellagicacid evidenced strong antiproliferative activity against bothC32 and A375 cells with IC50 values of 0.8 and 4.1 μg/ml,respectively. Interesting results were observed, also, withgallic acid that showed the highest cytotoxic activityagainst CaCo-2 (IC50 2.6 μg/ml). Overall, the results ofthis study suggest that D. lotus displays a good antioxidantactivity and has antiproliferative effects. Both activities arerelated to identified phenolic compounds.
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|Titolo:||Antioxidant and Antiproliferative Activity of Diospyros lotus L. Extract and Isolated Compounds|
|Data di pubblicazione:||2009|
|Appare nelle tipologie:||1.1 Articolo in rivista|