Fructose production by chicory inulin enzymatic hydrolysis: a kinetic study and reaction mechanism

In this paper, a reaction scheme for fructose production by inulin enzymatic hydrolysis is proposed, taking into account the possibility of dealing with a mixture of enzymes acting on a mixture of polymers as substrate. The scheme is subsequently simplified to obtain a stoichiometric relationship between the fructose product and the reacted substrate. The former may be measured by HPLC, while the latter is the subject of kinetic investigations. Our proposed kinetic model is defined within temperature and substrate concentration ranges of industrial interest (40–60 8C and 3–60 g/L, respectively). Some assumptions were made in order to simplify the model, which is based on a minimum number of parameters. These hypotheses were always specified and assumed only on the basis of convenience and rational consideration. Eventually, the kinetic model was successfully validated by comparison with a vast set of experimental results.