In our continuous search of biological properties of Senecio species (Compositae), we investigated S.ambiguus subsp. ambiguus (Biv.) DC, S. gibbosus subsp. gibbosus DC, S. leucanthemifolius Poiret, S. inaequidensDC, and S. vulgaris L. for their angiotensin converting enzyme (ACE) inhibitory activity through an invitro bioassay based on the enzymatic cleavage of the chromophore-fluorophore labelled substrate dansyltriglycineinto dansylglycine, which is quantitatively measured by HPLC. Among analyzed extracts, ethylacetate demonstrated the highest activity with IC50 values of 192.1 and 219.1 μg/mL for S. ambiguus subsp.ambiguus and S. inaequidens, respectively. Flavonoids were detected in these extracts on TLC sprayed withNatural Products reagent - polyethylene glycal reagent (NP/PEG).
Inhibition of angiotensin converting enzyme activity by five Senecio species
Loizzo M. R.;Tundis R.;Conforti F.;Statti G. A.;
2009-01-01
Abstract
In our continuous search of biological properties of Senecio species (Compositae), we investigated S.ambiguus subsp. ambiguus (Biv.) DC, S. gibbosus subsp. gibbosus DC, S. leucanthemifolius Poiret, S. inaequidensDC, and S. vulgaris L. for their angiotensin converting enzyme (ACE) inhibitory activity through an invitro bioassay based on the enzymatic cleavage of the chromophore-fluorophore labelled substrate dansyltriglycineinto dansylglycine, which is quantitatively measured by HPLC. Among analyzed extracts, ethylacetate demonstrated the highest activity with IC50 values of 192.1 and 219.1 μg/mL for S. ambiguus subsp.ambiguus and S. inaequidens, respectively. Flavonoids were detected in these extracts on TLC sprayed withNatural Products reagent - polyethylene glycal reagent (NP/PEG).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
