The yield of methanolic extract and total phenol and non polar content of flowered parts from Achillea ligustica ALL. are reported. GC-MS analysis of the non polar fraction showed that the triterpene moretenol was the major constituent (17.228%) followed by stigmast-6-en-3b -ol, veridiflorol and b -amyrin (7.524%, 5.078% 4.470%, respectively). The antioxidant activities of the methanolic extract and its fractions from A. ligustica were carried out using two different in vitro assays, 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and lipid peroxidation of liposomes assay. Methanolic extract showed higher radical scavenging activity on DPPH (IC50 of 50m g/ml). This activity is probably due to the phenolic fraction which shown an IC50 value of 22m g/ml. A different result was obtained from the methanolic extract on the lipid peroxidation of liposomes (IC50 of 416m g/ml). The a-amylase inhibition assay was applied to evaluate antidiabetic activity. The methanolic extract showed weak activity (28.18% at 1 mg/ml) while the n-hexane fraction showed 74.96% inhibition at 250m g/ml
COMPARATIVE RADICAL SCAVENGING AND ANTIDIABETIC ACTIVITIES OF METHANOLIC EXTRACT AND FRACTIONS FROM ACHILLEA LIGUSTICA ALL
CONFORTI, FILOMENA;LOIZZO, Monica Rosa;STATTI, Giancarlo;
2005-01-01
Abstract
The yield of methanolic extract and total phenol and non polar content of flowered parts from Achillea ligustica ALL. are reported. GC-MS analysis of the non polar fraction showed that the triterpene moretenol was the major constituent (17.228%) followed by stigmast-6-en-3b -ol, veridiflorol and b -amyrin (7.524%, 5.078% 4.470%, respectively). The antioxidant activities of the methanolic extract and its fractions from A. ligustica were carried out using two different in vitro assays, 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and lipid peroxidation of liposomes assay. Methanolic extract showed higher radical scavenging activity on DPPH (IC50 of 50m g/ml). This activity is probably due to the phenolic fraction which shown an IC50 value of 22m g/ml. A different result was obtained from the methanolic extract on the lipid peroxidation of liposomes (IC50 of 416m g/ml). The a-amylase inhibition assay was applied to evaluate antidiabetic activity. The methanolic extract showed weak activity (28.18% at 1 mg/ml) while the n-hexane fraction showed 74.96% inhibition at 250m g/mlI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.