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EnglishHuman SIRT3 gene contains an intronic VNTR enhancer. A T > C transition occurring in the second repeat of each VNTR allele implies the presence/absence of a putative GATA binding motif. A partially overlapping AP-1 site, not affected by the transition, was also identified. Aims of the present study were: 1) to verify if GATA and AP-1 sites could bind GATA2 and c-Jun/c-Fos factors, respectively; 2) to investigate whether such sites modulate the enhancer activity of the SIRT3-VNTR alleles. DAPA assay proved that GATA2 and c-Jun/c-Fos factors are able to bind the corresponding sites. Moreover, co-transfection experiments showed that the over-expression of GATA2 and c-Jun/c-Fos factors boosts the VNTR enhancer activity in an allelic-specific way. Furthermore, we established that GATA2 and c-Jun/c-Fos act additively in modulating the SIRT3-VNTR enhancer function. Therefore, GATA2 and AP-1 are functional sites and the T S > C transition of the second VNTR repeat affects their activity.
Identification of GATA2 and AP-1 Activator elements within the enhancer VNTR occurring in intron 5 of the human SIRT3 gene / Bellizzi, Dina; Covello, G; DI CIANNI, F; Tong, Q; DE BENEDICTIS, G.. - In: MOLECULES AND CELLS. - ISSN 1016-8478. - 28(2009), pp. 87-92.
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| Titolo: | Identification of GATA2 and AP-1 Activator elements within the enhancer VNTR occurring in intron 5 of the human SIRT3 gene |
| Autori: | |
| Data di pubblicazione: | 2009 |
| Rivista: | |
| Citazione: | Identification of GATA2 and AP-1 Activator elements within the enhancer VNTR occurring in intron 5 of the human SIRT3 gene / Bellizzi, Dina; Covello, G; DI CIANNI, F; Tong, Q; DE BENEDICTIS, G.. - In: MOLECULES AND CELLS. - ISSN 1016-8478. - 28(2009), pp. 87-92. |
| Handle: | http://hdl.handle.net/20.500.11770/130007 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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