Senecio ambiguus subsp. ambiguus (Biv.) DC. extracts were able to inhibit the in vitro proliferationof renal cell adenocarcinoma ACHN and hormone dependent prostate carcinomaLNCaP. The potential cytotoxic property of the plant was revealed by the methanolic extractaction against LNCaP (IC50 of 5.51 μg/mL) and ACHN (IC50 of 38.95 μg/mL). The most potentcytotoxic activity (IC50 of 5.34 μg/mL against the prostate carcinoma cell line) was exerted bythe dichloromethane extract. Through bioassay-guided fractionation of the dichloromethaneextract jacaranone was isolated as the major active constituent. This quinoid showed a verystrong activity against ACHN and LNcaP with IC50 of 4.32 and 7.39 μg/mL, respectively. Itsstructure was established by GC/MS and NMR analysis. The n-hexane extract showed aninteresting inhibition on the proliferation of tumor cell lines an IC50 value of 5.23 μg/mL againstLNCaP. Three compounds identified in the n-hexane extract such as nerolidol, alpha-humuleneand gamma-tocopherol were found to be active aginst LNCAP with IC50 values ranged from 11.24 to 15.56 μg/mL.
Jacaranone: a cytotoxic constituent from Senecio ambiguus subsp. ambiguus (Biv.) DC. against renal adenocarcinoma ACHN and prostate carcinoma LNCaP cells
LOIZZO, Monica Rosa;TUNDIS, ROSA;STATTI, Giancarlo;
2007-01-01
Abstract
Senecio ambiguus subsp. ambiguus (Biv.) DC. extracts were able to inhibit the in vitro proliferationof renal cell adenocarcinoma ACHN and hormone dependent prostate carcinomaLNCaP. The potential cytotoxic property of the plant was revealed by the methanolic extractaction against LNCaP (IC50 of 5.51 μg/mL) and ACHN (IC50 of 38.95 μg/mL). The most potentcytotoxic activity (IC50 of 5.34 μg/mL against the prostate carcinoma cell line) was exerted bythe dichloromethane extract. Through bioassay-guided fractionation of the dichloromethaneextract jacaranone was isolated as the major active constituent. This quinoid showed a verystrong activity against ACHN and LNcaP with IC50 of 4.32 and 7.39 μg/mL, respectively. Itsstructure was established by GC/MS and NMR analysis. The n-hexane extract showed aninteresting inhibition on the proliferation of tumor cell lines an IC50 value of 5.23 μg/mL againstLNCaP. Three compounds identified in the n-hexane extract such as nerolidol, alpha-humuleneand gamma-tocopherol were found to be active aginst LNCAP with IC50 values ranged from 11.24 to 15.56 μg/mL.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
