The transcription factor p73 belongs to the p53 family of tumour suppressors and similar to other familymembers, transcribed as different isoforms with opposing pro‐ and anti‐apoptotic functions. Unlike p53, p73 mutations areextremely rare in cancers. Instead, the pro‐apoptotic activities of transcriptionally active p73 isoforms are commonlyinhibited by over‐expression of the dominant negative p73 isoforms. Therefore the relative ratio of different p73 isoformsis critical for the cellular response to a chemotherapeutic agent. Here, we analysed the expression of N‐terminal p73isoforms in cell lines and mouse tissues. Our data showed that the transcriptionally competent TAp73 isoform isabundantly expressed in cancer cell lines compared to the dominant negative ΔNp73 isoform. Interestingly, we detectedhigher levels of ΔNp73 in some mouse tissues, suggesting that ΔNp73 may have a physiological role in these tissues.
Relative expression of TAp73 and ΔNp73 isoforms
TUCCI, Paola;
2012-01-01
Abstract
The transcription factor p73 belongs to the p53 family of tumour suppressors and similar to other familymembers, transcribed as different isoforms with opposing pro‐ and anti‐apoptotic functions. Unlike p53, p73 mutations areextremely rare in cancers. Instead, the pro‐apoptotic activities of transcriptionally active p73 isoforms are commonlyinhibited by over‐expression of the dominant negative p73 isoforms. Therefore the relative ratio of different p73 isoformsis critical for the cellular response to a chemotherapeutic agent. Here, we analysed the expression of N‐terminal p73isoforms in cell lines and mouse tissues. Our data showed that the transcriptionally competent TAp73 isoform isabundantly expressed in cancer cell lines compared to the dominant negative ΔNp73 isoform. Interestingly, we detectedhigher levels of ΔNp73 in some mouse tissues, suggesting that ΔNp73 may have a physiological role in these tissues.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
