This study aimed to investigate the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitoryactivity of the essential oils from Pinus nigra subsp. nigra, P. nigra var. calabrica, and P. heldreichii subsp. leucodermis.This activity is relevant to the treatment of Alzheimer’s disease (AD), since cholinesterase drugs are currently theonly drugs available to treat AD. P. heldreichii subsp. leucodermis exhibited the most promising activity, with IC50values of 51.1 and 80.6 μg/mL against AChE and BChE, respectively. An interesting activity against AChE was alsoobserved with P. nigra subsp. nigra essential oil, with an IC50 value of 94.4 μg/mL. Essential oils were analyzedby GC and GC-MS with the purpose of investigating their relationships with the observed activities. Among theidentified constituents, terpinolene, β-phellandrene, linalyl acetate, trans-caryophyllene, and terpinen-4-ol weretested. trans-Caryophyllene and terpinen-4-ol inhibited BChE with IC50 values of 78.6 and 107.6 μg/mL, respectively.β-Phellandrene was selective against AChE (IC50 value of 120.2 μg/mL).
Acetylcholinesterase and butyrylcholinesterase inhibitory activity of Pinus species essential oils and their constituents
Bonesi M;TUNDIS, ROSA;LOIZZO, Monica Rosa;CONFORTI, FILOMENA;Passalacqua NG;STATTI, Giancarlo;
2010
Abstract
This study aimed to investigate the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitoryactivity of the essential oils from Pinus nigra subsp. nigra, P. nigra var. calabrica, and P. heldreichii subsp. leucodermis.This activity is relevant to the treatment of Alzheimer’s disease (AD), since cholinesterase drugs are currently theonly drugs available to treat AD. P. heldreichii subsp. leucodermis exhibited the most promising activity, with IC50values of 51.1 and 80.6 μg/mL against AChE and BChE, respectively. An interesting activity against AChE was alsoobserved with P. nigra subsp. nigra essential oil, with an IC50 value of 94.4 μg/mL. Essential oils were analyzedby GC and GC-MS with the purpose of investigating their relationships with the observed activities. Among theidentified constituents, terpinolene, β-phellandrene, linalyl acetate, trans-caryophyllene, and terpinen-4-ol weretested. trans-Caryophyllene and terpinen-4-ol inhibited BChE with IC50 values of 78.6 and 107.6 μg/mL, respectively.β-Phellandrene was selective against AChE (IC50 value of 120.2 μg/mL).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
