This study showed for the first time the antioxidant and hypoglycaemicproperties of the methanol, n-hexane and ethyl acetate extracts fromSenecio stabianus Lacaita, a plant that belongs to the Asteraceae family.The antioxidant activities were carried out using two different in vitroassays, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and 2,20-azinobis(3-ethylbenzthiazoline-6-sulphonate) (ABTS) test. The ethylacetate extract showed the highest activity with inhibitory concentration50% (IC50) values of 35.5 and 32.7 mg/mL on DPPH test and ABTS test,respectively. This activity may be related to a good total phenol andflavonoid content. All extracts were also tested for their potential inhibitoryactivity of alpha-amylase and alpha-glucosidase digestive enzymes. The n-hexaneextract exhibited the highest alpha-amylase inhibition with an IC50 value of0.21mg/mL. Through bioassay-guided fractionation processes sevenfractions (A–G) were obtained and tested. Based on the phytochemicalanalysis, the activity of n-hexane extract may be related to the presence ofmonoterpenes and sesquiterpenes.
Studies on the potential antioxidant properties of Senecio stabianus Lacaita (Asteraceae) and its inhibitory activity against carbohydrate-hydrolysing enzymes
TUNDIS, ROSA;LOIZZO, Monica Rosa;Bonesi M;
2012-01-01
Abstract
This study showed for the first time the antioxidant and hypoglycaemicproperties of the methanol, n-hexane and ethyl acetate extracts fromSenecio stabianus Lacaita, a plant that belongs to the Asteraceae family.The antioxidant activities were carried out using two different in vitroassays, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and 2,20-azinobis(3-ethylbenzthiazoline-6-sulphonate) (ABTS) test. The ethylacetate extract showed the highest activity with inhibitory concentration50% (IC50) values of 35.5 and 32.7 mg/mL on DPPH test and ABTS test,respectively. This activity may be related to a good total phenol andflavonoid content. All extracts were also tested for their potential inhibitoryactivity of alpha-amylase and alpha-glucosidase digestive enzymes. The n-hexaneextract exhibited the highest alpha-amylase inhibition with an IC50 value of0.21mg/mL. Through bioassay-guided fractionation processes sevenfractions (A–G) were obtained and tested. Based on the phytochemicalanalysis, the activity of n-hexane extract may be related to the presence ofmonoterpenes and sesquiterpenes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
