The anions (I-III) obtained from O-methyl 5'-O-(5'-deoxythymidine) 3'-O-(2',3'-dideoxyuridine) phosphate by the competitive removal of the 3-N-H protons of the nucleobases and of the methyl group from the phosphotriester bond, assume in the gas phase stable conformations as a function of their charge site. The mass-analyzed ion kinetic energy (MIKE) spectra of I and III show that the regioselective backbone cleavage of the internucleotidic linkage is controlled by the 2'-H proton transfer to the nucleobase within the 5'-end nucleoside. Similar pathways are taken by species II when the nucleobase is eliminated as neutral from the 5'-end nucleoside. (C) 1997 American Society for Mass Spectrometry. OI sindona, giovanni/0000-0002-5623-5795; Liguori, Angelo/0000-0003-4483-2506

The anions (I-III) obtained from O-methyl 5'-O-(5'-deoxythymidine) 3'-O-(2',3'-dideoxyuridine) phosphate by the competitive removal of the 3-N-H protons of the nucleobases and of the methyl group from the phosphotriester bond, assume in the gas phase stable conformations as a function of their charge site. The mass-analyzed ion kinetic energy (MIKE) spectra of I and III show that the regioselective backbone cleavage of the internucleotidic linkage is controlled by the 2'-H proton transfer to the nucleobase within the 5'-end nucleoside. Similar pathways are taken by species II when the nucleobase is eliminated as neutral from the 5'-end nucleoside

Participation of the nucleobases in the regioselective backbone fragmentation of nucleic acids. A molecular dynamics and tandem mass spectrometric investigation on a model dinucleoside phosphotriester / Sindona, Giovanni; Liguori, Angelo; Maiuolo, L; Marino, Tiziana; Procopio, A.. - In: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY. - ISSN 1044-0305. - 32:12(1997), pp. 1257-1261.

Participation of the nucleobases in the regioselective backbone fragmentation of nucleic acids. A molecular dynamics and tandem mass spectrometric investigation on a model dinucleoside phosphotriester

De Nino A;SINDONA, Giovanni;LIGUORI, Angelo;MARINO, Tiziana;
1997

Abstract

The anions (I-III) obtained from O-methyl 5'-O-(5'-deoxythymidine) 3'-O-(2',3'-dideoxyuridine) phosphate by the competitive removal of the 3-N-H protons of the nucleobases and of the methyl group from the phosphotriester bond, assume in the gas phase stable conformations as a function of their charge site. The mass-analyzed ion kinetic energy (MIKE) spectra of I and III show that the regioselective backbone cleavage of the internucleotidic linkage is controlled by the 2'-H proton transfer to the nucleobase within the 5'-end nucleoside. Similar pathways are taken by species II when the nucleobase is eliminated as neutral from the 5'-end nucleoside. (C) 1997 American Society for Mass Spectrometry. OI sindona, giovanni/0000-0002-5623-5795; Liguori, Angelo/0000-0003-4483-2506
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11770/133246
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