p73 is a p53 family transcription factor. Due to the presence in the 5’ flanking region of twopromoters, there are two N-terminal variants, TAp73 that retains a fully active transactivationdomain (TA) and ΔNp73 in which the N-terminus is truncated. In addition, extensive 3’-splicinggives rise to at least seven distinctive isoforms TAp73 selective knockout highlights its role as aregulator of cell death, senescence and tumor suppressor. ΔNp73 selective knockout, on the otherhand, highlights anti-apoptotic function of ΔNp73 and its involvement in DNA damage response.In this work we investigated the expression pattern of murine p73 C-terminal isoforms. By using aRT-PCR approach we were able to detect mRNAs of all the C-terminal isoforms described inhuman. We characterized their in vivo expression profile in mouse organs and in different mousedevelopmental stages. Finally, we investigated p73 C-terminal expression profile following DNAdamage: ex vivo after primary cultures treatment and in vivo after systemic administration ofcytotoxic compounds. Overall, our study first elucidates spatio-temporal expression of mouse p73isoforms and provides novel insights on their expression-switch under triggered conditions.

Tissue specific expression of p73 C-terminal isoforms in mice

TUCCI, Paola;
2012

Abstract

p73 is a p53 family transcription factor. Due to the presence in the 5’ flanking region of twopromoters, there are two N-terminal variants, TAp73 that retains a fully active transactivationdomain (TA) and ΔNp73 in which the N-terminus is truncated. In addition, extensive 3’-splicinggives rise to at least seven distinctive isoforms TAp73 selective knockout highlights its role as aregulator of cell death, senescence and tumor suppressor. ΔNp73 selective knockout, on the otherhand, highlights anti-apoptotic function of ΔNp73 and its involvement in DNA damage response.In this work we investigated the expression pattern of murine p73 C-terminal isoforms. By using aRT-PCR approach we were able to detect mRNAs of all the C-terminal isoforms described inhuman. We characterized their in vivo expression profile in mouse organs and in different mousedevelopmental stages. Finally, we investigated p73 C-terminal expression profile following DNAdamage: ex vivo after primary cultures treatment and in vivo after systemic administration ofcytotoxic compounds. Overall, our study first elucidates spatio-temporal expression of mouse p73isoforms and provides novel insights on their expression-switch under triggered conditions.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11770/134342
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