The NADPH-dependent geranylgeranyl reductase gene (OeCHLP) was characterised in olive (Oleaeuropaea L.). OeCHLP catalyses the formation of carbon double bonds in the phytolic side chain of chlorophyll,tocopherols and plastoquinones and, therefore, is involved in metabolic pathways related to plant productivity andstress response, besides to nutritional value of its products. The nuclear OeCHLP encodes a deduced product of51 kDa, which harbours a transit peptide for cytoplasm-to-chloroplast transport and a nicotinamide binding domain.Two estimated identical copies of gene are harboured per haploid genome of the cv. ‘Carolea’ used in the present study.Levels and cytological pattern of OeCHLP transcription were investigated by quantitative RT–PCR and in situhybridisation. In line with the presence of ubiquitous tocopherols and/or chlorophyll, OeCHLP transcripts were presentin various organs of plants. In leaves and fruits at different developmental stages, OeCHLP was differentially expressed inrelation to their morpho-physiological features. An early and transient enhancement of gene transcription was detected inleaves of different age exposed to cold treatment (4C), as well as in fruits mechanically wounded. Moreover, OeCHLPtranscripts locally increased in specific cell domains of fruits severely damaged by the pathogen Bactrocera olea. Combined,these data show that OeCHLP expression early responds to biotic and abiotic stressful factors. Levels of tocopherols alsoincreased in leaves exposed to cold conditions and fruits severely damaged by pathogen.Wesuggest that gene activity understress condition could be related to tocopherol action.
The NADPH-dependent geranylgeranyl reductase gene (OeCHLP) was characterised in olive (Oleaeuropaea L.). OeCHLP catalyses the formation of carbon double bonds in the phytolic side chain of chlorophyll,tocopherols and plastoquinones and, therefore, is involved in metabolic pathways related to plant productivity andstress response, besides to nutritional value of its products. The nuclear OeCHLP encodes a deduced product of51 kDa, which harbours a transit peptide for cytoplasm-to-chloroplast transport and a nicotinamide binding domain.Two estimated identical copies of gene are harboured per haploid genome of the cv. ‘Carolea’ used in the present study.Levels and cytological pattern of OeCHLP transcription were investigated by quantitative RT–PCR and in situhybridisation. In line with the presence of ubiquitous tocopherols and/or chlorophyll, OeCHLP transcripts were presentin various organs of plants. In leaves and fruits at different developmental stages, OeCHLP was differentially expressed inrelation to their morpho-physiological features. An early and transient enhancement of gene transcription was detected inleaves of different age exposed to cold treatment (4C), as well as in fruits mechanically wounded. Moreover, OeCHLPtranscripts locally increased in specific cell domains of fruits severely damaged by the pathogen Bactrocera olea. Combined,these data show that OeCHLP expression early responds to biotic and abiotic stressful factors. Levels of tocopherols alsoincreased in leaves exposed to cold conditions and fruits severely damaged by pathogen.Wesuggest that gene activity understress condition could be related to tocopherol action.
Role of geranylgeranyl reductase gene in organ development and stress response in olive (Olea europaea L) plants
Bruno L.;Chiappetta A.;Muzzalupo I.;Bitonti M. B.
2009-01-01
Abstract
The NADPH-dependent geranylgeranyl reductase gene (OeCHLP) was characterised in olive (Oleaeuropaea L.). OeCHLP catalyses the formation of carbon double bonds in the phytolic side chain of chlorophyll,tocopherols and plastoquinones and, therefore, is involved in metabolic pathways related to plant productivity andstress response, besides to nutritional value of its products. The nuclear OeCHLP encodes a deduced product of51 kDa, which harbours a transit peptide for cytoplasm-to-chloroplast transport and a nicotinamide binding domain.Two estimated identical copies of gene are harboured per haploid genome of the cv. ‘Carolea’ used in the present study.Levels and cytological pattern of OeCHLP transcription were investigated by quantitative RT–PCR and in situhybridisation. In line with the presence of ubiquitous tocopherols and/or chlorophyll, OeCHLP transcripts were presentin various organs of plants. In leaves and fruits at different developmental stages, OeCHLP was differentially expressed inrelation to their morpho-physiological features. An early and transient enhancement of gene transcription was detected inleaves of different age exposed to cold treatment (4C), as well as in fruits mechanically wounded. Moreover, OeCHLPtranscripts locally increased in specific cell domains of fruits severely damaged by the pathogen Bactrocera olea. Combined,these data show that OeCHLP expression early responds to biotic and abiotic stressful factors. Levels of tocopherols alsoincreased in leaves exposed to cold conditions and fruits severely damaged by pathogen.Wesuggest that gene activity understress condition could be related to tocopherol action.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
