A comparative study of phytochemicals content and biological properties of eightRosmarinus officinalis (rosemary) populations (RO1–RO8) collected in different areas ofTunisia was carried out. Two of the main rosemary constituents, rosmarinic and carnosicacids, were quantified by an NMR technique. Carnosic acid content was higher than thatof rosmarinic acid. The non-polar constituents were examined by GC and GC–MS. Totalphenols and flavonoids content were also determined in order to discuss the possiblecorrelation between these phytochemicals and bioactivity. Antioxidant activity was investigatedthrough different in vitro assays. Sample RO3 from a sub-humid area showed thehighest potency in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay (IC50 of 6.74 microg/mL) whileRO5 from arid area exhibited the highest radical 2,20-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) scavenging activity (IC50 of 5.92 microg/mL). The most active sample inFerric Reducing Antioxidant Power (FRAP) assay was RO4 (62.21 microM Fe(II)/g), followed byRO1–RO3 and RO8 harvested in sub-humid areas with values in the range 49.61–58.72 microMFe(II)/g. Extracts were able to inhibit acethylcholinesterase (AChE) and butyrylcholinesterase(BChE). Sample RO5 exhibited a promising AChE inhibitory activity (IC50 of 4.47 microg/mL). Thus, we suggest rosemary extracts as a functional food ingredient for preventing AD.
NMR-based quantification of rosmarinic and carnosic acids, GC-MS profile and bioactivity relevant to neurodegenerative disorders of Rosmarinus officinalis L. extracts
Tundis R.;Loizzo M. R.
2013-01-01
Abstract
A comparative study of phytochemicals content and biological properties of eightRosmarinus officinalis (rosemary) populations (RO1–RO8) collected in different areas ofTunisia was carried out. Two of the main rosemary constituents, rosmarinic and carnosicacids, were quantified by an NMR technique. Carnosic acid content was higher than thatof rosmarinic acid. The non-polar constituents were examined by GC and GC–MS. Totalphenols and flavonoids content were also determined in order to discuss the possiblecorrelation between these phytochemicals and bioactivity. Antioxidant activity was investigatedthrough different in vitro assays. Sample RO3 from a sub-humid area showed thehighest potency in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay (IC50 of 6.74 microg/mL) whileRO5 from arid area exhibited the highest radical 2,20-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) scavenging activity (IC50 of 5.92 microg/mL). The most active sample inFerric Reducing Antioxidant Power (FRAP) assay was RO4 (62.21 microM Fe(II)/g), followed byRO1–RO3 and RO8 harvested in sub-humid areas with values in the range 49.61–58.72 microMFe(II)/g. Extracts were able to inhibit acethylcholinesterase (AChE) and butyrylcholinesterase(BChE). Sample RO5 exhibited a promising AChE inhibitory activity (IC50 of 4.47 microg/mL). Thus, we suggest rosemary extracts as a functional food ingredient for preventing AD.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
