The use of sweeteners extracted from leaves of the plant species Stevia Rebaudiana is worldwide increasing. They are recognized as GRAS (Generally Recognized as Safe) by US-FDA and approved by EU-EFSA, with some recommendation on the daily dosage that should not interfere with glucose metabolism. The results here presented introduce an easy analytical approach for the identification and assay of Stevia sweeteners in commercially available soft drink, based on Liquid Chromatography (LC) coupled to Tandem Mass Spectrometry (MS/MS), using a natural statin like molecule, Brutieridin, as internal standard.
L’uso di dolcificanti estratti da foglie della specie Stevia Rebaudiana è in continuo aumento a livello mondiale. Tali molecole sono classificate come GRAS dalla FDA americana ed approvate dall’EFSA europea, con la raccomandazione che la dose giornaliera non deve interferire con il metabolismo del glucosio. I risultati qui presentati introducono un semplice approccio analitico per la identificazione ed il saggio di dolcificanti della Stevia in bibite commerciali, basato sulla Cromatografia Liquida (LC) accoppiata alla spettrometria di massa tandem (MS/MS), facendo uso di una molecola statino-simile, la Brutieridina, come standard interno.
Tandem Mass Spectrometry: A Convenient Approach in the Dosage of Steviol Glycosides in Stevia Sweetened Commercial Food Beverages
DI DONNA, Leonardo;MAZZOTTI, Fabio;SINDONA, Giovanni
2017-01-01
Abstract
The use of sweeteners extracted from leaves of the plant species Stevia Rebaudiana is worldwide increasing. They are recognized as GRAS (Generally Recognized as Safe) by US-FDA and approved by EU-EFSA, with some recommendation on the daily dosage that should not interfere with glucose metabolism. The results here presented introduce an easy analytical approach for the identification and assay of Stevia sweeteners in commercially available soft drink, based on Liquid Chromatography (LC) coupled to Tandem Mass Spectrometry (MS/MS), using a natural statin like molecule, Brutieridin, as internal standard.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.