tIn this study, stearic acid- and stearyl ferulate-based solid lipid nanoparticles containing trans-ferulic acid(SLN-FA and SLN-SF-FA, respectively), were prepared and characterized for loading efficiency, size andshape. In addition, by using rat brain microsomes, we evaluated in vitro the antioxidant activity of theseformulations against three well known initiators of lipid peroxidation, such as AAPH, NADPH/ADP-Fe3+and SIN-1 which in turn generate the peroxyl and perferryl radicals as well as peroxynitrite, respectively.Commercially available FA and its ethyl ester (FAEE) were used as comparators. Both SLN-FA and SLN-SF-FA dose-dependently reduced lipid peroxidation induced by the three oxidants. Interestingly, SLN-SF-FA displayed greater efficacy (EC50) and potency (maximal activity) against AAPH- and NADPH/ADP-Fe3+-induced lipid peroxidation. Our results support the idea that this new formulations could facilitatethe uptake of FA by the cells because of their lipophilic structure, thus increasing FA bioavailability.Furthermore, stearyl ferulate-based nanoparticles could prevent the degradation of FA entrapped ontheir structure, making FA almost entirely available to explicate its antioxidant power once released
Trans-ferulic acid-based solid lipid nanoparticles and their antioxidant effect in rat brain microsomes
TROMBINO, Sonia;CASSANO, Roberta;PICCI, Nevio;
2013-01-01
Abstract
tIn this study, stearic acid- and stearyl ferulate-based solid lipid nanoparticles containing trans-ferulic acid(SLN-FA and SLN-SF-FA, respectively), were prepared and characterized for loading efficiency, size andshape. In addition, by using rat brain microsomes, we evaluated in vitro the antioxidant activity of theseformulations against three well known initiators of lipid peroxidation, such as AAPH, NADPH/ADP-Fe3+and SIN-1 which in turn generate the peroxyl and perferryl radicals as well as peroxynitrite, respectively.Commercially available FA and its ethyl ester (FAEE) were used as comparators. Both SLN-FA and SLN-SF-FA dose-dependently reduced lipid peroxidation induced by the three oxidants. Interestingly, SLN-SF-FA displayed greater efficacy (EC50) and potency (maximal activity) against AAPH- and NADPH/ADP-Fe3+-induced lipid peroxidation. Our results support the idea that this new formulations could facilitatethe uptake of FA by the cells because of their lipophilic structure, thus increasing FA bioavailability.Furthermore, stearyl ferulate-based nanoparticles could prevent the degradation of FA entrapped ontheir structure, making FA almost entirely available to explicate its antioxidant power once releasedI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
