Role of 14-3-3 proteins in the regulation of neutral trehalase in the yeast Saccharomyces cerevisiae

Panni, Simona; Landgraf, C; Volkmer Engert, R; Cesareni, G; Castagnoli, L.

doi:DOI:10.1111/j.1567-1364.2007.00312.x

In higher eukaryotes, 14-3-3 proteins participate in numerous cellular processes, and carry out their function through a variety of different molecular mechanisms, including regulation of protein localization and enzyme activation. Here, it is shown that the two yeast 14-3-3 homologues, Bmh1p and Bmh2p, form a complex with neutral trehalase (Nth1p), an enzyme that is responsible for trehalose degradation and is required in a variety of stress conditions. In a purified in vitro system, either one of the two 14-3-3 yeast isoforms are necessary for complete activation of neutral trehalase (Nth1p) after phosphorylation by PKA. It is further demonstrated that Bmh1p and Bmh2p bind to the amino-terminal region of phosphorylated trehalase, thereby modulating its enzymatic activity. This work represents the first demonstration of enzyme activation mediated by 14-3-3 binding in yeast.

Role of 14-3-3 proteins in the regulation of neutral trehalase in the yeast Saccharomyces cerevisiae / Panni, Simona; Landgraf, C; VOLKMER ENGERT, R; Cesareni, G; Castagnoli, L.. - In: FEMS YEAST RESEARCH. - ISSN 1567-1356. - 8(2008), pp. 53-63.

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Titolo:	Role of 14-3-3 proteins in the regulation of neutral trehalase in the yeast Saccharomyces cerevisiae
Autori:	PANNI, Simona LANDGRAF C VOLKMER ENGERT R CESARENI G CASTAGNOLI L. mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2008
Rivista:	FEMS YEAST RESEARCH
Citazione:	Role of 14-3-3 proteins in the regulation of neutral trehalase in the yeast Saccharomyces cerevisiae / Panni, Simona; Landgraf, C; VOLKMER ENGERT, R; Cesareni, G; Castagnoli, L.. - In: FEMS YEAST RESEARCH. - ISSN 1567-1356. - 8(2008), pp. 53-63.
Handle:	http://hdl.handle.net/20.500.11770/143795
Appare nelle tipologie:	1.1 Articolo in rivista

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