The quality of extra virgin olive oil is associatedwith the presence of microcomponents whose healing effectshave been proved in some special cases. The enzymatic hydrolysisof oleuropein and ligstroside, and of their demethylatedanalogues, affords four different pentanedialdehydes, and forone of which, 2-(4-hydroxyphenyl)ethyl (3S,4E)-4-formyl-3-(2-oxoethyl)hex-4-enoate, also known as oleocanthal, ananti-inflammatory effect was quite recently carefully assessed.Extra virgin olive oil is now worldwide considered as a functionalfood whose daily intake, as for the Mediterranean diet,helps consumers in keeping a constant level of nonsteroidal anti-inflammatory drug (NSAID) in the blood. The presence of theseactive principles provides, therefore, olive oil with an important added value. In the framework of the actions of the recently fundedAgrifood Regional Center, which should coordinate the scientific research and production worlds, an absolute analytical method wasdeveloped for the mass spectrometric detection of the two most abundant NSAIDs, Tyr-OLPD and HTyr-OLPD(oleopentanedialdehydes (OLPDs) conjugated to p-hydroxyphenylethanol and 3,4-dihydroxyphenylethanol, respectively), byUHPLC-ESI-MS/MS.

The quality of extra virgin olive oil is associated with the presence of microcomponents whose healing effects have been proved in some special cases. The enzymatic hydrolysis of oleuropein and ligstroside, and of their demethylated analogues, affords four different pentanedialdehydes, and for one of which, 2-(4-hydroxyphenyl)ethyl (3S,4E)-4-formyl-3-(2-oxoethyl)hex-4-enoate, also known as oleocanthal, an anti-inflammatory effect was quite recently carefully assessed. Extra virgin olive oil is now worldwide considered as a functional food whose daily intake, as for the Mediterranean diet, helps consumers in keeping a constant level of nonsteroidal anti-inflammatory drug (NSAID) in the blood. The presence of these active principles provides, therefore, olive oil with an important added value. In the framework of the actions of the recently funded Agrifood Regional Center, which should coordinate the scientific research and production worlds, an absolute analytical method was developed for the mass spectrometric detection of the two most abundant NSAIDs, Tyr-OLPD and HTyr-OLPD (oleopentanedialdehydes (OLPDs) conjugated to p-hydroxyphenylethanol and 3,4-dihydroxyphenylethanol, respectively), by UHPLC-ESI-MS/MS.

High-Throughput Assay of Oleopentanedialdheydes in Extra Virgin Olive Oil by the UHPLC-ESI-MS/MS and Isotope Dilution Methods

DI DONNA, Leonardo;MAZZOTTI, Fabio;NAPOLI, Anna Maria Carmela Natale V;SINDONA, Giovanni
2011

Abstract

The quality of extra virgin olive oil is associated with the presence of microcomponents whose healing effects have been proved in some special cases. The enzymatic hydrolysis of oleuropein and ligstroside, and of their demethylated analogues, affords four different pentanedialdehydes, and for one of which, 2-(4-hydroxyphenyl)ethyl (3S,4E)-4-formyl-3-(2-oxoethyl)hex-4-enoate, also known as oleocanthal, an anti-inflammatory effect was quite recently carefully assessed. Extra virgin olive oil is now worldwide considered as a functional food whose daily intake, as for the Mediterranean diet, helps consumers in keeping a constant level of nonsteroidal anti-inflammatory drug (NSAID) in the blood. The presence of these active principles provides, therefore, olive oil with an important added value. In the framework of the actions of the recently funded Agrifood Regional Center, which should coordinate the scientific research and production worlds, an absolute analytical method was developed for the mass spectrometric detection of the two most abundant NSAIDs, Tyr-OLPD and HTyr-OLPD (oleopentanedialdehydes (OLPDs) conjugated to p-hydroxyphenylethanol and 3,4-dihydroxyphenylethanol, respectively), by UHPLC-ESI-MS/MS.
The quality of extra virgin olive oil is associatedwith the presence of microcomponents whose healing effectshave been proved in some special cases. The enzymatic hydrolysisof oleuropein and ligstroside, and of their demethylatedanalogues, affords four different pentanedialdehydes, and forone of which, 2-(4-hydroxyphenyl)ethyl (3S,4E)-4-formyl-3-(2-oxoethyl)hex-4-enoate, also known as oleocanthal, ananti-inflammatory effect was quite recently carefully assessed.Extra virgin olive oil is now worldwide considered as a functionalfood whose daily intake, as for the Mediterranean diet,helps consumers in keeping a constant level of nonsteroidal anti-inflammatory drug (NSAID) in the blood. The presence of theseactive principles provides, therefore, olive oil with an important added value. In the framework of the actions of the recently fundedAgrifood Regional Center, which should coordinate the scientific research and production worlds, an absolute analytical method wasdeveloped for the mass spectrometric detection of the two most abundant NSAIDs, Tyr-OLPD and HTyr-OLPD(oleopentanedialdehydes (OLPDs) conjugated to p-hydroxyphenylethanol and 3,4-dihydroxyphenylethanol, respectively), byUHPLC-ESI-MS/MS.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11770/144546
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