Citrus x limon cv. Femminello Comune (Rutaceae) from Rocca Imperiale (Italy), one of the six Protected GeographicalIndication (PGI) Italian lemon crops, has been recently received renewed interest. In this work, fresh and dried peels andleaves were extracted by hydrodistillation, supercritical fluid extraction (SFE), and Soxhlet apparatus. Chemical profile wasassessed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Except for leaves extractsobtained by Soxhlet apparatus, the monoterpene hydrocarbons fraction dominated. Limonene, c-terpinene, and b-pinene werethe main identified compounds. The antioxidant activity was investigated using different in vitro assays namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS, ferric reducing ability power (FRAP), and b-carotene bleaching test. In DPPH test, theessential oil obtained by hydrodistillation of fresh peel exhibited the highest activity (IC50 of 1.17 mg/ml). Leaves extracted bySFE showed a good activity in both DPPH and b-carotene bleaching test with IC50 values of 2.20 and 6.66 mg/ml,respectively. Monoterpene hydrocarbons fraction exhibited a positive Pearson’s correlation coefficient with all antioxidantassays. Leaves, often considered waste material, should be considered from a different point because they represent a matrixof indisputable interest.
Chemical profile and antioxidant properties of extracts and essential oils from Citrus x limon (L.) Burm. cv Femminello comune
Loizzo M. R.;Tundis R.
;Bonesi M.;Verardi A.;Lopresto C. G.;
2016-01-01
Abstract
Citrus x limon cv. Femminello Comune (Rutaceae) from Rocca Imperiale (Italy), one of the six Protected GeographicalIndication (PGI) Italian lemon crops, has been recently received renewed interest. In this work, fresh and dried peels andleaves were extracted by hydrodistillation, supercritical fluid extraction (SFE), and Soxhlet apparatus. Chemical profile wasassessed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Except for leaves extractsobtained by Soxhlet apparatus, the monoterpene hydrocarbons fraction dominated. Limonene, c-terpinene, and b-pinene werethe main identified compounds. The antioxidant activity was investigated using different in vitro assays namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS, ferric reducing ability power (FRAP), and b-carotene bleaching test. In DPPH test, theessential oil obtained by hydrodistillation of fresh peel exhibited the highest activity (IC50 of 1.17 mg/ml). Leaves extracted bySFE showed a good activity in both DPPH and b-carotene bleaching test with IC50 values of 2.20 and 6.66 mg/ml,respectively. Monoterpene hydrocarbons fraction exhibited a positive Pearson’s correlation coefficient with all antioxidantassays. Leaves, often considered waste material, should be considered from a different point because they represent a matrixof indisputable interest.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
