An investigationwasconductedtodeterminetheeffectsoftheheavymetal,cadmium(Cd),onthegills of theteleostfish, Thalassomapavo Linnaeus,1758.Thefisheswereexposedtoseveralsublethal concentrationsofcadmium(10,40,60and120 mM (mg/L))foraperiodof48,96and192h.Thevalueof the LC50after96hofcadmiumexposure,determinedusingtheSystemofFinney,wasequalto 128.3 mM. Thegillsofthefisheswereexaminedbylightandelectronmicroscopy.Toxic,apoptoticand cadmiumeffectswereanalyzedusingsomeneuropeptides,metallothioneins(MT),caspase3,PCNAand calmodulin,asbioindicators,respectively.Theresultsshowedthatthealterationsinthegillswere proportional totheexposureperiodsandconcentrationsofthemetal,whichwerefoundtobebothdose and timedependent.Thebiologicalresponsesinthegillsofthetestedanimalsarediscussedinrelation to resultsobtainedbyanalysisofthebiomarkers.Thesedatamaybeusedfortheplanningofamodelto determine biologicalriskinthemarineenvironmentandmaybeparticularlyusefultoinvestigate organismsexposedtocadmium.
Ultrastructural and immunohistochemical investigation on the gills of the teleost, Thalassoma pavo L., exposed to cadmium
BRUNELLI, Elvira
Project Administration
;Bernabo', I;Corapi, B;TRIPEPI, S;
2011-01-01
Abstract
An investigationwasconductedtodeterminetheeffectsoftheheavymetal,cadmium(Cd),onthegills of theteleostfish, Thalassomapavo Linnaeus,1758.Thefisheswereexposedtoseveralsublethal concentrationsofcadmium(10,40,60and120 mM (mg/L))foraperiodof48,96and192h.Thevalueof the LC50after96hofcadmiumexposure,determinedusingtheSystemofFinney,wasequalto 128.3 mM. Thegillsofthefisheswereexaminedbylightandelectronmicroscopy.Toxic,apoptoticand cadmiumeffectswereanalyzedusingsomeneuropeptides,metallothioneins(MT),caspase3,PCNAand calmodulin,asbioindicators,respectively.Theresultsshowedthatthealterationsinthegillswere proportional totheexposureperiodsandconcentrationsofthemetal,whichwerefoundtobebothdose and timedependent.Thebiologicalresponsesinthegillsofthetestedanimalsarediscussedinrelation to resultsobtainedbyanalysisofthebiomarkers.Thesedatamaybeusedfortheplanningofamodelto determine biologicalriskinthemarineenvironmentandmaybeparticularlyusefultoinvestigate organismsexposedtocadmium.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
