In the course of recent efforts to identify new potential antiproliferative active principles, Salvialeriifolia extracts and isolated constituents were evaluated for their cytotoxic activity against a panel ofhuman cancer cell lines, including renal adenocarcinoma (ACHN), amelanotic melanoma (C32),colorectal adenocarcinoma (Caco-2), lung large cell carcinoma (COR-L23), malignant melanoma(A375), lung carcinoma (A549), and hepatocellular carcinoma (Huh-7D12) cells. The hexane andCH2Cl2 extracts showed the strongest cytotoxic activity against the C32 cell line with IC50 values of 11.2and 13.6 mg/ml, respectively, and the AcOEt extract was the most active extract against the COR-L23 cellline (IC50 of 20.9 mg/ml). Buchariol, a sesquiterpene obtained by biofractionation of the CH2Cl2 extract,exhibited a higher activity than the positive control vinblastine against the C32 and A549 cell lines (IC50values of 2.1 and 12.6 mm, resp.). Interesting results were also obtained for naringenin, a flavonoidisolated from the AcOEt extract, which exhibited a strong cytotoxic activity against the C32, LNCaP, andCOR-L23 cell lines (IC50 values of 2.2, 7.7, and 33.4 mm, resp.), compared to vinblastine (IC50 values of3.3, 32.2, 50.0 mm, resp.). None of the tested compounds affected the proliferation of skin fibroblasts(142BR), suggesting a selective activity against tumor cells.
In vitro cytotoxic activity of extracts and isolated constituents of salvia leriifolia benth. Against a panel of human cancer cell lines
Tundis R.;Loizzo M. R.;Bonesi M.;
2011-01-01
Abstract
In the course of recent efforts to identify new potential antiproliferative active principles, Salvialeriifolia extracts and isolated constituents were evaluated for their cytotoxic activity against a panel ofhuman cancer cell lines, including renal adenocarcinoma (ACHN), amelanotic melanoma (C32),colorectal adenocarcinoma (Caco-2), lung large cell carcinoma (COR-L23), malignant melanoma(A375), lung carcinoma (A549), and hepatocellular carcinoma (Huh-7D12) cells. The hexane andCH2Cl2 extracts showed the strongest cytotoxic activity against the C32 cell line with IC50 values of 11.2and 13.6 mg/ml, respectively, and the AcOEt extract was the most active extract against the COR-L23 cellline (IC50 of 20.9 mg/ml). Buchariol, a sesquiterpene obtained by biofractionation of the CH2Cl2 extract,exhibited a higher activity than the positive control vinblastine against the C32 and A549 cell lines (IC50values of 2.1 and 12.6 mm, resp.). Interesting results were also obtained for naringenin, a flavonoidisolated from the AcOEt extract, which exhibited a strong cytotoxic activity against the C32, LNCaP, andCOR-L23 cell lines (IC50 values of 2.2, 7.7, and 33.4 mm, resp.), compared to vinblastine (IC50 values of3.3, 32.2, 50.0 mm, resp.). None of the tested compounds affected the proliferation of skin fibroblasts(142BR), suggesting a selective activity against tumor cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.