The selection of appropriate biomaterials that promote cellular adhesion and growth is particularly important for the in vitro reconstruction of neuronal network. This study focused on the develop- ment of new polymeric mem- branes in flat and tubular (hollow-fibre) configurations as novel biomaterials for neuronal outgrowth. Two membrane systems constituted by modified polyetherether-ketone (PEEK-WC) and poly- acrylonitrile (PAN) membranes were developed and used for the culture of hamster hippocampal neurons. We demonstrated that all investigated membranes supported the adhesion and growth of hippocampal neurons enhancing neuronal differen-tiation and neurite align- ment. The differences in cell behaviours between cells cultured on flat and hollow- fibre (HF) membranes were highlighted by the quantita- tive analysis of neuronal marker fluorescence inten- sity, morphometric analysis, RT –PCR analysis and also by metabolic activity measure-ments. In particular, the PAN HF membranes showed ideal growth culture conditions, guaranteeing adequate levels of metabolic features. Primary hippocampal cells cultured on PAN HF membranes were able to recreate in vitro a 3D neural tissue-like structure that, mimicking the hippocampal tissue, could be used as a tool for the study of natural and pathological neurobiological events.

Flat and tubular membrane systems for the reconstruction of hippocampal neuronal network

CANONACO, Marcello;
2012

Abstract

The selection of appropriate biomaterials that promote cellular adhesion and growth is particularly important for the in vitro reconstruction of neuronal network. This study focused on the develop- ment of new polymeric mem- branes in flat and tubular (hollow-fibre) configurations as novel biomaterials for neuronal outgrowth. Two membrane systems constituted by modified polyetherether-ketone (PEEK-WC) and poly- acrylonitrile (PAN) membranes were developed and used for the culture of hamster hippocampal neurons. We demonstrated that all investigated membranes supported the adhesion and growth of hippocampal neurons enhancing neuronal differen-tiation and neurite align- ment. The differences in cell behaviours between cells cultured on flat and hollow- fibre (HF) membranes were highlighted by the quantita- tive analysis of neuronal marker fluorescence inten- sity, morphometric analysis, RT –PCR analysis and also by metabolic activity measure-ments. In particular, the PAN HF membranes showed ideal growth culture conditions, guaranteeing adequate levels of metabolic features. Primary hippocampal cells cultured on PAN HF membranes were able to recreate in vitro a 3D neural tissue-like structure that, mimicking the hippocampal tissue, could be used as a tool for the study of natural and pathological neurobiological events.
hollow fibre; neuronal culture; neuronal tissue engineering
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11770/156774
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