Phytochemical analysis and in vitro evaluation of the biological activity against herpes simplex virus type 1 (HSV-1) of Cedrus libani A Rich

Cedrus libani are widely used as traditional medicine in Lebanon for treatment of different infection diseases. In thepresent study we reported the phytochemical composition analyzed by GC–MS of wood essential oil and cones andleaves ethanol extracts. The main components of wood essential oil were himachalol (22.50%), b-himachalene(21.90%), and a-himachalene (10.50%). Leaves ethanol extract was characterized by a high content of germacrene D(29.40%). The same extract obtained from cones essentially contained a-pinene (51.0%) and b-myrcene (13.0%).Moreover, we investigated extracts, essential oil, and identified compounds for their in vitro antiviral activities againstherpes simplex virus type 1 (HSV-1). Cytotoxicity was evaluated by MTT assay in Vero cells. Cones and leaves ethanolextracts exhibited an interesting activity with IC50 of 0.50 and 0.66 mg/ml, respectively, at non-cytotoxic concentration.A comparable activity was found when essential oil was tested (IC50 of 0.44 mg/ml).