The coordination of amino acids ortho-tyrosine (o-Tyr, 1) and trans-4-hydroxyproline (HPro, 2) with the biologically important trivalent metal ions Al(III) and Fe(III) in aqueous solution was investigated by1H and13C high resolution NMR, Laser Desorption Mass Spectrometry (LD-MS), and MS/MS experiments. Potentiometric measurements were also carried out in 0.16 M NaCl, 37 °C, by varying the pH between 2.0 and 3.5. NMR spectra recorded on aqueous solutions of amino acids 1 or 2 in the presence of the appropriate trivalent metal chloride suggested that binding of Al(III) and Fe(III) involved the COOH and NH2functional groups of ligands, while their phenolic and alcoholic groups which did not participate in the metal coordination. Potentiometry indicated the prevalent formation of complexes having the general stoichiometry ML and ML2. Laser desorption mass spectrometry (LD-MS) and MS/MS investigation proved the isotopic compositions of ML and ML2complexes, demonstrating their structures.

Study of the coordination of ortho-tyrosine and trans-4-hydroxyproline with aluminum(III) and iron(III)

Aiello, Donatella;Furia, Emilia;Siciliano, Carlo
;
Napoli, Anna
2018-01-01

Abstract

The coordination of amino acids ortho-tyrosine (o-Tyr, 1) and trans-4-hydroxyproline (HPro, 2) with the biologically important trivalent metal ions Al(III) and Fe(III) in aqueous solution was investigated by1H and13C high resolution NMR, Laser Desorption Mass Spectrometry (LD-MS), and MS/MS experiments. Potentiometric measurements were also carried out in 0.16 M NaCl, 37 °C, by varying the pH between 2.0 and 3.5. NMR spectra recorded on aqueous solutions of amino acids 1 or 2 in the presence of the appropriate trivalent metal chloride suggested that binding of Al(III) and Fe(III) involved the COOH and NH2functional groups of ligands, while their phenolic and alcoholic groups which did not participate in the metal coordination. Potentiometry indicated the prevalent formation of complexes having the general stoichiometry ML and ML2. Laser desorption mass spectrometry (LD-MS) and MS/MS investigation proved the isotopic compositions of ML and ML2complexes, demonstrating their structures.
2018
Electronic, Optical and Magnetic Materials; Atomic and Molecular Physics, and Optics; Condensed Matter Physics; Spectroscopy; Physical and Theoretical Chemistry; Materials Chemistry2506 Metals and Alloys
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11770/285396
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 19
social impact