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EnglishPrevious studies have pointed to the protective role of genistein against stress adaptations despite neuromolecular mechanisms are not yet fully known. With this work, we evaluated the influence of such a phytoestrogen on hamster behavioral and molecular activities following exposure to sub-chronic unpredictable mild stress (sCUMS). Methods: Motor behaviors of hamsters (n = 28) were analyzed using elevated plus-maze (EPM), hole board test (HB) and forced swim test (FST). In addition, neurodegeneration events were assessed with amino cupric silver stain (ACS) while expression variations of tropomyosin receptor-B kinase (TrkB), nuclear factor kappa- light-chain-enhancer of activated B1 cells (NF-kB1), and heat shock protein 70 (Hsp70) mRNAs were highlighted in limbic neuronal fields via in situ hybridization (ISH). Conclusion: Genistein accounted for increased motor performances in EPM and HB, but reduced immobility during FST, which were correlated with diminished argyrophilic signals in some limbic neuronal fields. Contextually, up-regulated Hsp70 and TrkB mRNAs occurred in hippocampal and hypothalamic neuronal fields. Conversely, diminished NF-kB1 levels were mainly obtained in the hippocampus. Hormonal neuroprotective properties of genistein corroborating anxiolytic and antidepressant role(s) through elevated expression levels of stress proteins and trophic factors, may constitute novel therapeutic measures against emotional and stressful-related motor performances.
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| Titolo: | Genistein modifies hamster behavioral and expression of inflammatory factors following sub-chronic unpredictable mild stress |
| Autori: | |
| Data di pubblicazione: | 2019 |
| Rivista: | |
| Abstract: | Previous studies have pointed to the protective role of genistein against stress adaptations despite neuromolecular mechanisms are not yet fully known. With this work, we evaluated the influence of such a phytoestrogen on hamster behavioral and molecular activities following exposure to sub-chronic unpredictable mild stress (sCUMS). Methods: Motor behaviors of hamsters (n = 28) were analyzed using elevated plus-maze (EPM), hole board test (HB) and forced swim test (FST). In addition, neurodegeneration events were assessed with amino cupric silver stain (ACS) while expression variations of tropomyosin receptor-B kinase (TrkB), nuclear factor kappa- light-chain-enhancer of activated B1 cells (NF-kB1), and heat shock protein 70 (Hsp70) mRNAs were highlighted in limbic neuronal fields via in situ hybridization (ISH). Conclusion: Genistein accounted for increased motor performances in EPM and HB, but reduced immobility during FST, which were correlated with diminished argyrophilic signals in some limbic neuronal fields. Contextually, up-regulated Hsp70 and TrkB mRNAs occurred in hippocampal and hypothalamic neuronal fields. Conversely, diminished NF-kB1 levels were mainly obtained in the hippocampus. Hormonal neuroprotective properties of genistein corroborating anxiolytic and antidepressant role(s) through elevated expression levels of stress proteins and trophic factors, may constitute novel therapeutic measures against emotional and stressful-related motor performances. |
| Handle: | http://hdl.handle.net/20.500.11770/289413 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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