Use of Galactose Oxidase in Hydrazide-Capturing Technique to Isolate N-linked Glycoallergens from Olive Tree Pollen

In this work, a simple method hydrazide-capturing technique followed by MALDI MS and MS/MS analysis for characterization of major pollen allergens of Olea europaea pollen was exploited. The strategy developed has different stages: devising of sample preparation protocol to sequester glycoproteins from a crude extract, and allowing the identification of antigenic peptides, glycosylation sites and modified glycans by MALDI MS and MS/MS.



Titolo: Use of Galactose Oxidase in Hydrazide-Capturing Technique to Isolate N-linked Glycoallergens from Olive Tree Pollen
Autori: 
NAPOLI, Anna Maria Carmela Natale Vittoria (Corresponding)
Data di pubblicazione: 2020
Rivista: 
IOP CONFERENCE SERIES: MATERIALS SCIENCE AND ENGINEERING  
Handle: http://hdl.handle.net/20.500.11770/299668
Appare nelle tipologie:1.1 Articolo in rivista

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http://hdl.handle.net/20.500.11770/299668
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