The ornithine/citrulline carrier from rat liver mitochondira, solubilized with Triton X‐100 and partially purified on hydroxyapatite, was identified and completely purified by PD‐10, DEAE‐Sephacel and celite chromatography. On SDS/polyacrylamide gel electrophoresis, the purified ornithine/citrulline carrier consisted of a single protein band with an apparent molecular mass of 33.5 kDa. When reconstituted into liposomes the ornithine carrier protein catalyzed an active mersalyl sensitive ornithine/ornithine exchange. It was purified 438‐fold with a recovery of 38% and a protein yield of 0.09% with respect to the extract derived from mitoplasts. The purified and reconstituted protein did not catalyze a significant unidirectional transport of ornithine. Citrulline was found to be the best countersubstrate for the transport of ornithine, followed by lysine and arginine. The exchange activity was inhibited by several sulphydryl reagents. Copyright © 1992, Wiley Blackwell. All rights reserved

Identification and purification of the ornithine/citrulline carrier from rat liver mitochondira

INDIVERI C.;
1992

Abstract

The ornithine/citrulline carrier from rat liver mitochondira, solubilized with Triton X‐100 and partially purified on hydroxyapatite, was identified and completely purified by PD‐10, DEAE‐Sephacel and celite chromatography. On SDS/polyacrylamide gel electrophoresis, the purified ornithine/citrulline carrier consisted of a single protein band with an apparent molecular mass of 33.5 kDa. When reconstituted into liposomes the ornithine carrier protein catalyzed an active mersalyl sensitive ornithine/ornithine exchange. It was purified 438‐fold with a recovery of 38% and a protein yield of 0.09% with respect to the extract derived from mitoplasts. The purified and reconstituted protein did not catalyze a significant unidirectional transport of ornithine. Citrulline was found to be the best countersubstrate for the transport of ornithine, followed by lysine and arginine. The exchange activity was inhibited by several sulphydryl reagents. Copyright © 1992, Wiley Blackwell. All rights reserved
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11770/310423
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