A hybrid fluorescent mesoporous silica material has been prepared starting from a mesoporous silica obtained by neutral surfactants. Synthesis was carried out according to a new interfacial procedure based on a not-catalyzed tetraethylorthosilicate hydrolysis aimed to tailor the particle size of the final product. As-synthesized materials have been derivatized with aminopropyl function partially coupled with fluoresceine isothiocyianate, a fluorescent tracer. Successively folic acid, a receptor-specific ligand has been covalently coupled to free aminopropyl groups. The resulting multifunctional material has been suspended in MCF-7 and HeLa cell cultures. Confocal microscopy showed cellular internalization in both cell lines, probably due to endocytosis. © 2007 Elsevier B.V. All rights reserved.
Preparation and cell inclusion of fluorescent folic acid- functionalizedmesoporous silica microspheres potentially useful for drug targeting
Pasqua L.;Morelli C.;Testa F.;Sisci D.;Brunelli E.;
2007-01-01
Abstract
A hybrid fluorescent mesoporous silica material has been prepared starting from a mesoporous silica obtained by neutral surfactants. Synthesis was carried out according to a new interfacial procedure based on a not-catalyzed tetraethylorthosilicate hydrolysis aimed to tailor the particle size of the final product. As-synthesized materials have been derivatized with aminopropyl function partially coupled with fluoresceine isothiocyianate, a fluorescent tracer. Successively folic acid, a receptor-specific ligand has been covalently coupled to free aminopropyl groups. The resulting multifunctional material has been suspended in MCF-7 and HeLa cell cultures. Confocal microscopy showed cellular internalization in both cell lines, probably due to endocytosis. © 2007 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
