In the legend of Fig. 6, panel B, of the above paper, the temperature at which the thermal shift assay was performed is missing. The sentence Fluorescent signal upon membrane solubilization with 1% Dodecyl-β-D-Maltopyranoside (DDM) and denaturation at the indicated temperature, was replaced by the sentence Fluorescent signal upon membrane solubilization with 1% Dodecyl-β-D-Maltopyranoside (DDM) and denaturation at the temperature of 51.8 °C. The correct legend is in full below: Figure 6. Cholesterol-dependent Thermal Stability of OCT2 Mutants. Membrane fractions were isolated from HEK293 cells transiently transfected with eGFP-WT, eGFP-R234A or eGFP-R263A constructs, exposed for 20 min to cholesterol-saturated methyl-β-cyclodextrin (RAMEB) at the extracellular concentration of 2.5 mM. (A) Total cellular cholesterol content resolved in a silica plate and quantification from the membrane fractions used for the thermal stability assay. (B) Fluorescent signal upon membrane solubilization with 1% Dodecyl-β-D-Maltopyranoside (DDM) and denaturation at the temperature of 51.8 °C. Values are relative to the fluorescent signal of the non-heated controls. Thermal stability experiment was performed three times, each with a minimum of two technical replicates, from the same membrane preparations. All p-values were calculated from two-way analysis of variance followed by Tukey's post-hoc test. The authors apologise for this error.

Corrigendum to “The role of cholesterol recognition (CARC/CRAC) mirror codes in the allosterism of the human organic cation transporter 2 (OCT2, SLC22A2)” [Biochem. Pharmacol. 194 (2021) 114840](S0006295221004561)(10.1016/j.bcp.2021.114840)

Console L.;Indiveri C.;
2022-01-01

Abstract

In the legend of Fig. 6, panel B, of the above paper, the temperature at which the thermal shift assay was performed is missing. The sentence Fluorescent signal upon membrane solubilization with 1% Dodecyl-β-D-Maltopyranoside (DDM) and denaturation at the indicated temperature, was replaced by the sentence Fluorescent signal upon membrane solubilization with 1% Dodecyl-β-D-Maltopyranoside (DDM) and denaturation at the temperature of 51.8 °C. The correct legend is in full below: Figure 6. Cholesterol-dependent Thermal Stability of OCT2 Mutants. Membrane fractions were isolated from HEK293 cells transiently transfected with eGFP-WT, eGFP-R234A or eGFP-R263A constructs, exposed for 20 min to cholesterol-saturated methyl-β-cyclodextrin (RAMEB) at the extracellular concentration of 2.5 mM. (A) Total cellular cholesterol content resolved in a silica plate and quantification from the membrane fractions used for the thermal stability assay. (B) Fluorescent signal upon membrane solubilization with 1% Dodecyl-β-D-Maltopyranoside (DDM) and denaturation at the temperature of 51.8 °C. Values are relative to the fluorescent signal of the non-heated controls. Thermal stability experiment was performed three times, each with a minimum of two technical replicates, from the same membrane preparations. All p-values were calculated from two-way analysis of variance followed by Tukey's post-hoc test. The authors apologise for this error.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11770/343176
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