OBJECTIVE - To test whether weight loss may improve endothelial dysfunction in human obesity, we recruited 28 healthy obese subjects, aged 30-46 years, with BMI 30-43 kg/m2. RESEARCH DESIGN AND METHODS - Endothelium-dependent and -independent vasodilation were investigated by intra-arterial infusion of increasing doses of acetylcholine (ACh; 7.5, 15, and 30 μg·ml-1·min-1) and sodium nitroprusside (0.8, 1.6, and 3.2 μg·ml-1 ·min-1). Insulin resistance was estimated by homeostasis model assessment (HOMA). Weight loss was obtained by caloric restriction and physical activity. RESULTS - We observed a significant reduction in BMI (from 33.1 ± 4.2 to 27.5 ± 4.5 kg/m2, - 16.9%, P < 0.0001) 1) and in waist circumference (from 108.2 ± 12.1 to 96.8 ± 12.9 cm, - 10.5%, P < 0.0001). Weight loss was also associated with a significant increase in ACh-stimulated forearm blood flow (FBF), from 7.4 ± 2.8 to 12.9 ± 3.4 ml-1·100 ml-1 of tissue · min-1 kg/m2 (P < 0.0001). Multivariate regression analysis demonstrated that the only independent predictor of FBF was HOMA, accounting for 44.5% of the variation, whereas the addition of BMI explained another 2.3% of the variation. CONCLUSIONS - Our data demonstrate that energy-restricted diet associated with physical activity induce a significant and clinically relevant improvement in ACh-stimulated vasodilation in obese healthy subjects.

Weight loss in combination with physical activity improves endothelial dysfunction in human obesity

Scozzafava, A.;Corsonello, A.;
2003-01-01

Abstract

OBJECTIVE - To test whether weight loss may improve endothelial dysfunction in human obesity, we recruited 28 healthy obese subjects, aged 30-46 years, with BMI 30-43 kg/m2. RESEARCH DESIGN AND METHODS - Endothelium-dependent and -independent vasodilation were investigated by intra-arterial infusion of increasing doses of acetylcholine (ACh; 7.5, 15, and 30 μg·ml-1·min-1) and sodium nitroprusside (0.8, 1.6, and 3.2 μg·ml-1 ·min-1). Insulin resistance was estimated by homeostasis model assessment (HOMA). Weight loss was obtained by caloric restriction and physical activity. RESULTS - We observed a significant reduction in BMI (from 33.1 ± 4.2 to 27.5 ± 4.5 kg/m2, - 16.9%, P < 0.0001) 1) and in waist circumference (from 108.2 ± 12.1 to 96.8 ± 12.9 cm, - 10.5%, P < 0.0001). Weight loss was also associated with a significant increase in ACh-stimulated forearm blood flow (FBF), from 7.4 ± 2.8 to 12.9 ± 3.4 ml-1·100 ml-1 of tissue · min-1 kg/m2 (P < 0.0001). Multivariate regression analysis demonstrated that the only independent predictor of FBF was HOMA, accounting for 44.5% of the variation, whereas the addition of BMI explained another 2.3% of the variation. CONCLUSIONS - Our data demonstrate that energy-restricted diet associated with physical activity induce a significant and clinically relevant improvement in ACh-stimulated vasodilation in obese healthy subjects.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11770/344503
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