OBJECTIVE: To examine whether the developmental potential of embryos that were partially damaged after freezing and thawing can be improved by removal of necrotic blastomeres before embryo transfer.DESIGN: Prospective pilot study and observational clinical series.SETTING: Private hospital.PATIENT(S): Two hundred thirty-five infertile couples undergoing frozen embryo transfer.INTERVENTION(S): Removal of necrotic blastomeres from frozen-thawed human embryos.MAIN OUTCOME MEASURE(S): Pregnancy and implantation rates.RESULT(S): Removal of necrotic blastomeres from partially damaged frozen-thawed embryos before transfer increased rates of pregnancy (45.7% vs. 17.1%), ongoing pregnancy (40.0% vs. 11.4%) and ongoing implantation (16.2% vs. 4.3%) compared with the control group, in which necrotic blastomeres were not removed. A similarly high implantation rate (16.7%) was seen a subsequent clinical series in which necrotic blastomeres were removed from all partially damaged embryos.CONCLUSION(S): The viability of partially damaged frozen-thawed embryos can be improved by removal of necrotic blastomeres before embryo transfer.
Laser-assisted removal of necrotic blastomeres from cryopreserved embryos that were partially damaged.
Ubaldi F;
2002-01-01
Abstract
OBJECTIVE: To examine whether the developmental potential of embryos that were partially damaged after freezing and thawing can be improved by removal of necrotic blastomeres before embryo transfer.DESIGN: Prospective pilot study and observational clinical series.SETTING: Private hospital.PATIENT(S): Two hundred thirty-five infertile couples undergoing frozen embryo transfer.INTERVENTION(S): Removal of necrotic blastomeres from frozen-thawed human embryos.MAIN OUTCOME MEASURE(S): Pregnancy and implantation rates.RESULT(S): Removal of necrotic blastomeres from partially damaged frozen-thawed embryos before transfer increased rates of pregnancy (45.7% vs. 17.1%), ongoing pregnancy (40.0% vs. 11.4%) and ongoing implantation (16.2% vs. 4.3%) compared with the control group, in which necrotic blastomeres were not removed. A similarly high implantation rate (16.7%) was seen a subsequent clinical series in which necrotic blastomeres were removed from all partially damaged embryos.CONCLUSION(S): The viability of partially damaged frozen-thawed embryos can be improved by removal of necrotic blastomeres before embryo transfer.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
