Bergamot Essential Oil (BEO, Citrus bergamia Risso et Poiteau) proved strong, preclinical evidence of efficacy in models of pain relevant to clinical conditions (Scuteri, Hamamura, et al. 2021), fundamentally involved in the development of agitation during dementia (Sampson et al. 2015) when misidentified (Sengstaken and King 1993), and the role of its components after administration by inhalation (Scuteri, Rombolà, Crudo, et al. 2022b) and transdermal route (Scuteri, Rombolà, Crudo, et al. 2022a) was investigated. Due to its proven effectiveness, BEO deprived of bergapten was engineered in a nanotechnology delivery system, based on solid lipid nanoparticles (SLN), and devised in an odorless cream known as NanoBEO (Scuteri, Cassano, et al. 2021) (patent n° 102019000013353; international patent deposit n° EP 4003294) for clinical study in the frame of the pilot phase of the trial BRAINAID (NCT04321889) (Scuteri, Sandrini, et al. 2021). NanoBEO confirmed the properties of BEO (Scuteri, Rombolà, Hayashi, et al. 2022), but the pharmacokinetics (PK) characterization of active components after application is necessary to deepen the knowledge concerned with the reported biological and phenotypical effects (Scuteri et al. 2024). Previous studies developed feasible and accurate gas chromatography–mass spectroscopy (GC–MS) methods for the PK determination and quantification of volatile constituents in animal plasma after EOs administration (Friedl et al. 2010; Li et al. 2018; Wang et al. 2023). Thus, the aim of the present study was to adapt a simple, fast, and reliable GC–MS method for the simultaneous determination of limonene, linalool, and linalyl acetate in plasma samples to allow the PK profile of these volatile compounds to be established after transdermal administration of NanoBEO.
Gas Chromatography–Mass Spectroscopy ( GC – MS ) Simultaneous Determination of Limonene, Linalool, and Linalyl Acetate in Rat Plasma Following Transdermal Administration of the Essential Oil of Bergamot Loaded Onto Solid Lipid Nanoparticles ( NanoBEO )
Morrone, Luigi A.
;Leggio, Antonella;Belsito, Emilia L.;Scorzafave, Ludovica;De Rasis, Enrica;Pagliaro, Martina;Bagetta, Giacinto;Corasaniti, Maria Tiziana;Scuteri, Damiana
2025-01-01
Abstract
Bergamot Essential Oil (BEO, Citrus bergamia Risso et Poiteau) proved strong, preclinical evidence of efficacy in models of pain relevant to clinical conditions (Scuteri, Hamamura, et al. 2021), fundamentally involved in the development of agitation during dementia (Sampson et al. 2015) when misidentified (Sengstaken and King 1993), and the role of its components after administration by inhalation (Scuteri, Rombolà, Crudo, et al. 2022b) and transdermal route (Scuteri, Rombolà, Crudo, et al. 2022a) was investigated. Due to its proven effectiveness, BEO deprived of bergapten was engineered in a nanotechnology delivery system, based on solid lipid nanoparticles (SLN), and devised in an odorless cream known as NanoBEO (Scuteri, Cassano, et al. 2021) (patent n° 102019000013353; international patent deposit n° EP 4003294) for clinical study in the frame of the pilot phase of the trial BRAINAID (NCT04321889) (Scuteri, Sandrini, et al. 2021). NanoBEO confirmed the properties of BEO (Scuteri, Rombolà, Hayashi, et al. 2022), but the pharmacokinetics (PK) characterization of active components after application is necessary to deepen the knowledge concerned with the reported biological and phenotypical effects (Scuteri et al. 2024). Previous studies developed feasible and accurate gas chromatography–mass spectroscopy (GC–MS) methods for the PK determination and quantification of volatile constituents in animal plasma after EOs administration (Friedl et al. 2010; Li et al. 2018; Wang et al. 2023). Thus, the aim of the present study was to adapt a simple, fast, and reliable GC–MS method for the simultaneous determination of limonene, linalool, and linalyl acetate in plasma samples to allow the PK profile of these volatile compounds to be established after transdermal administration of NanoBEO.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
