An efficient and general solution-phase method for the site-specific N-methylation of peptides hasbeen developed. This novel procedure involves synthesis of N-nosyl protected peptides and theirsubsequent N-methylation with diazomethane. Its efficiency was proved by the successful synthesisof various hindered oligopeptides containing N-methyl amino acid residues with excellent yieldand purity. The method is particularly attractive in that the adopted conditions do not cause anydetectable racemization of the peptide stereocenters and the process does not require chromatographicpurification of the methylated products. A further advantage is the compatibility of thismethodology with Fmoc solution-phase peptide synthesis.

N-Methylation of Peptides on Selected Positions during the Elongation of the Peptide Chain in Solution Phase

DI GIOIA, Maria Luisa;LEGGIO, Antonella;LIGUORI, Angelo
2005-01-01

Abstract

An efficient and general solution-phase method for the site-specific N-methylation of peptides hasbeen developed. This novel procedure involves synthesis of N-nosyl protected peptides and theirsubsequent N-methylation with diazomethane. Its efficiency was proved by the successful synthesisof various hindered oligopeptides containing N-methyl amino acid residues with excellent yieldand purity. The method is particularly attractive in that the adopted conditions do not cause anydetectable racemization of the peptide stereocenters and the process does not require chromatographicpurification of the methylated products. A further advantage is the compatibility of thismethodology with Fmoc solution-phase peptide synthesis.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11770/124338
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 30
  • ???jsp.display-item.citation.isi??? 30
social impact