The separation and determination of triacylglycerols (TAGs), which are the main componentsof naturally occurring fats and oils, in milk fat is a challenging task due to thevery complex nature of this matrix. In the present study the TAG fraction of donkeymilk lipids has been characterized by using high performance liquid chromatographyatmosphericpressure chemical ionization mass spectrometry (HPLC-APCI-MS).HPLC in reversed phase mode has been used for TAG separation and silver ion (Ag+)HPLC has been used as a second dimension to clarify and confirm the identification.The RP-HPLC eluate was fractionated and the fractions of interest were injected ontothe Ag+-HPLC column. In both cases peak assignment was carried out by combiningretention data with APCI-MS spectra information. In total, 55 TAGs in donkey milk fatwere identified (without considering the positional isomers) and quantified on thebasis of percentage peak areas in the RP-HPLC chromatogram (without the use ofcorrection factors). Amongst the identified triacylglycerols, POLn, POO, PPO, CaPO,POL, and PPoO proved to be the main components of the TAG fraction of donkeymilk.
Determination of triacylglycerols in donkey milk by using high performance liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry
FAZIO, Alessia;
2005-01-01
Abstract
The separation and determination of triacylglycerols (TAGs), which are the main componentsof naturally occurring fats and oils, in milk fat is a challenging task due to thevery complex nature of this matrix. In the present study the TAG fraction of donkeymilk lipids has been characterized by using high performance liquid chromatographyatmosphericpressure chemical ionization mass spectrometry (HPLC-APCI-MS).HPLC in reversed phase mode has been used for TAG separation and silver ion (Ag+)HPLC has been used as a second dimension to clarify and confirm the identification.The RP-HPLC eluate was fractionated and the fractions of interest were injected ontothe Ag+-HPLC column. In both cases peak assignment was carried out by combiningretention data with APCI-MS spectra information. In total, 55 TAGs in donkey milk fatwere identified (without considering the positional isomers) and quantified on thebasis of percentage peak areas in the RP-HPLC chromatogram (without the use ofcorrection factors). Amongst the identified triacylglycerols, POLn, POO, PPO, CaPO,POL, and PPoO proved to be the main components of the TAG fraction of donkeymilk.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
