Background: The polyphenols derived from red wine, as the catechin, possesshighly antihypertensive properties so the possibility of their use in vitro isstudied since they prevent oxidative stress-induced endothelial dysfunction.The aim of this work is to examine the role of the reactive oxygen and nitricoxide species (ROSs/RNSs) in the damage induced by hypothermia inHUVECs considering also the interaction with Ang II, Losartan, PD123319.Materials and Methods:Authors analyzed the effect of the Cathechin (50μM) and of the Angiotensin II, Losartan, PD123319 both in normal and ininjured cells at long times of culture. After thermal damage at temperaturesof 33-34° the cells were incubated with different dyes: Acridine Orangefor vitality test, 2¹7¹-diclorodiidrofluoresceina diacetate for the evaluationof ROS production, the DAF-2DA for RNSs and Propidium Iodide for testedmolecular damage on DNA of Huvecs. The images were captured by Cell-Aprogram on Olympus IX 50 microscope and intensity of fluorescence wasevaluated by NIH Image J program. Results:The data obtained show afterhypotermic shock : proliferation increase in samples treated with Ang IIand PD123319; decreases the production of ROS for the samples treated withAng II, Losartan and PD123319 and Catechins; decreases of NO production inall treated samples (Ang II and Catechin), increases of nuclear damage but notin presence of Catechins.
Effect of the catechin in the mechanism of production / degradation of ROS and RNS in hypothermia on HUVEC
BRUNO, Rosalinda;MAZZULLA S;MARTINO G.
2010-01-01
Abstract
Background: The polyphenols derived from red wine, as the catechin, possesshighly antihypertensive properties so the possibility of their use in vitro isstudied since they prevent oxidative stress-induced endothelial dysfunction.The aim of this work is to examine the role of the reactive oxygen and nitricoxide species (ROSs/RNSs) in the damage induced by hypothermia inHUVECs considering also the interaction with Ang II, Losartan, PD123319.Materials and Methods:Authors analyzed the effect of the Cathechin (50μM) and of the Angiotensin II, Losartan, PD123319 both in normal and ininjured cells at long times of culture. After thermal damage at temperaturesof 33-34° the cells were incubated with different dyes: Acridine Orangefor vitality test, 2¹7¹-diclorodiidrofluoresceina diacetate for the evaluationof ROS production, the DAF-2DA for RNSs and Propidium Iodide for testedmolecular damage on DNA of Huvecs. The images were captured by Cell-Aprogram on Olympus IX 50 microscope and intensity of fluorescence wasevaluated by NIH Image J program. Results:The data obtained show afterhypotermic shock : proliferation increase in samples treated with Ang IIand PD123319; decreases the production of ROS for the samples treated withAng II, Losartan and PD123319 and Catechins; decreases of NO production inall treated samples (Ang II and Catechin), increases of nuclear damage but notin presence of Catechins.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
